ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Copying and
Applying Detectors
to the Plate
Document
4-8 Run Setup and Basic Operation
To create detectors for the plate document: (continued)
Step Action
8 Click OK to save the detector and return to the Detector Manager dialog box.
The software saves the new detector and displays it in the detector list.
9 Repeat steps 2 to 8 to create detectors for all remaining assays on the plate.
Note Click the button for information on the features of the Detector Manager
10
dialog box or to view the procedures for editing, deleting, or searching for detectors.
Choose from the following:
If constructing a
plate document for… Then…
absolute quantification copy the detector(s) to the plate document as
explained in the procedure below.
allelic discrimination create markers to the plate document as explained
on page 4-9.
IMPORTANT Once copied to the plate document, a detector is no longer linked to the
corresponding entry in the Detector Manager. Therefore, if a detector is modified in the Detector
Manager after it has been copied to a plate document, the detector must be removed from the
plate and copied again to update the plate document with the changes.
To copy and assign the detectors to the plate document:
Step Action
1 From the Detector Manager dialog box of the SDS software, copy the detectors to
the plate document as follows:
a. While pressing and holding the Ctrl key, click the detectors you want to apply to
the plate document.
The software highlights the selected detectors.
b. Click Copy to Plate Document.
The software adds the detectors to the well inspector of the plate document.
2 Click Done to close the Detector Manager dialog box.
3 From the plate grid, select the wells containing the assay for the first detector.
Note For easier selection of plate grid wells, use the Ctrl and Shift keys to select
wells individually or in groups. See page 3-16 for more information.
4 Apply detector to the selection by clicking the check box for the detector in the Use
column of the well inspector.
Use check box
Detector added to selected
wells of the plate document
5 Repeat steps 3 and 4 to apply the remaining detectors to the plate grid.
6 Configure the plate document with detector tasks as explained on page 4-11.