ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

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Consumables and Disposables The ABI PRISM 7900HT Sequence Detection System can run both: ♦ ABI PRISM Optical 384-Well Reaction Plates sealed with ABI PRISM Optical Adhesive Covers ♦ ABI PRISM 96-Well Reaction Plates sealed with ABI PRISM Optical Adhesive Covers or ABI PRISM® Optical Caps (flat cap strips only) IMPORTANT Do not use MicroAmp ® Optical Caps or MicroAmp ® Optical Tubes with the 7900HT instrument. The instrument is not designed to run MicroAmp consumables which may damage its internal components if used. Note ABI PRISM Optical Reaction Plates are designed specifically for fluorescence-based PCR chemistries and are frosted to minimize external fluorescent contamination. Before running prepared ABI PRISM Optical Reaction Plates on the 7900HT instrument, each plate must be sealed with an ABI PRISM Optical Adhesive Cover. Applied Biosystems’ optical adhesive covers are specifically designed to permit the transmission of light to and from the wells of the optical plate. Part No. Description Quantity ABI PRISM Optical Adhesive Covers 4313663 ABI PRISM Optical Adhesive Cover Starter Kit 20 Covers Includes 20 ABI PRISM Optical Adhesive Covers, an Applicator, and a ABI PRISM Optical Cover Compression Pad. 4311971 ABI PRISM Optical Adhesive Covers 100 Covers 4323032 ABI PRISM Optical Caps, 8 Caps/Strip 300 Strips/ Pkg 384-Well Optical Reaction Plates 4309849 ABI PRISM 384-Well Clear Optical Reaction Plate with Barcode (code 128) 4326270 10-Pack, ABI PRISM 384-Well Clear Optical Reaction Plate with Barcode (code 128) Includes 10 ABI PRISM 384-Well Clear Optical Reaction Plate with Barcode (P/N 4309849). 96-Well Optical Reaction Plates 4306737 ABI PRISM 96-Well Optical Reaction Plate with Barcode (code 128) 4326659 25-Pack, ABI PRISM 96-Well Optical Reaction Plate with Barcode (code 128) Includes 25 ABI PRISM 96-Well Optical Reaction Plate with Barcode (P/N 4306737). 4314320 ABI PRISM 96-Well Optical Reaction Plate with Barcode (code 128) and ABI PRISM Optical Adhesive Covers Includes 100 ABI PRISM Optical Adhesive Covers (P/N 4311971) and 5 ABI PRISM 96-Well Optical Reaction Plate with Barcode packages (P/N 4306737). Miscellaneous 4312063 MicroAmp® Splash Free Support Base for 96-Well Reaction Plates 2400 Caps/ Pkg 50 Plates 500 Plates 20 Plates 500 Plates 100 Plates 100 Covers 10 Bases Kits, Reagents and Consumables D-3
Instrument Maintenance and Verification D-4 Kits, Reagents and Consumables The following sequence detection kits and reagents are used to perform routine maintenance on and verify the function of the ABI PRISM 7900HT Sequence Detection System. For more information about the use of the kits below, see Chapter 7, “System Maintenance.” Part Number Description Quantity Sequence Detection Systems Spectral Calibration Kits 4328639 ABI PRISM 7900HT Sequence Detection Systems 96-Well Spectral Calibration Kit Includes three ABI PRISM Optical 384-Well Reaction Plates: one preloaded and sealed Background plate, and two preloaded and sealed Spectral Calibration plates containing eight separate dye standards (FAM, JOE, NED, ROX, SYBR® Green, TAMRA, TET, VIC). 4323977 Sequence Detection Systems 384-Well Spectral Calibration Kit Includes two ABI PRISM Optical 384-Well Reaction Plates: one preloaded and sealed Background plate, and one preloaded and sealed Spectral Calibration plate containing eight separate dye standards (FAM, JOE, NED, ROX, SYBR Green, TAMRA, TET, VIC). TaqMan RNase P Instrument Verification Plates 4310982 TaqMan® RNase P Instrument Verification Plate Includes one ABI PRISM Optical 96-well Reaction Plate pre-loaded and sealed with Sequence Detection primers and TaqMan® probe to detect and quantitate genomic copies of the human RNase P gene. 4323306 TaqMan® RNase P 384-Well Instrument Verification Plate Includes one ABI PRISM Optical 384-well Reaction Plate preloaded and sealed with complete Sequence Detection primers and TaqMan® probe to detect and quantitate genomic copies of the human RNase P gene. 3x96-Well Plates 2 x 384-Well Plates 1x96-Well Plate 1 x 384-Well Plate
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ABI PRISM ® 7900HT Sequence Detect
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© Copyright 2001, Applied Biosyste
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iv Section:PlateDocumentSetup......
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vi A Theory of Operation Fluorescen
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Attention Words and Warning Labels
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About Waste Profiles A waste profil
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Safe Instrument Use Before Operatin
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Product Overview 2 In This Chapter
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Section: Getting to Know the Hardwa
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Interchangeable Thermal Cycler Bloc
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Computer Description The computer c
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Zymark Twister Microplate Handler D
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Instrument Connections Electrical C
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Section: Getting to Know the Softwa
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Managing Sequence Detection System
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Getting Started 3 In This Chapter T
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Using the SDS Software Online Help
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Turning on the ABI PRISM 7900HT Seq
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Using the SDS Software Workspace La
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Using the General Toolbar Using the
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Basic Software Skills Tutorial Abou
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Exercise 3: Opening a Plate Documen
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Lesson 2: Viewing and Resizing Pane
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Exercise 2: Selecting Multiple Well
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Lesson 4: Using the Hand-Held Bar C
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Using SDS Plate Documents Using Mul
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Run Setup and Basic Operation 4 In
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Setup Checklists Experiments/Runs P
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Section: Plate Document Setup In Th
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Step 2 - Applying Detectors and Mar
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Creating an Allelic Discrimination
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Step 3 - Configuring the Plate Docu
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Step 4 - Programming the Plate Docu
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To create a method for the absolute
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Step 5 - Saving the Plate Document
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Step 7 - Applying Sample and Plate
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Section: Running an Individual Plat
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Preparing and Running a Single Plat
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Operating the 7900HT Instrument Usi
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After the Run Analyzing the Run Dat
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Section: Running Multiple Plates Us
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Adding a Plate Document to the Plat
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To create plate documents from a te
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Adding Plates to the Plate Queue Re
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Loading Plates To load plates onto
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End-Point Analysis 5 In This Chapte
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Section: Allelic Discrimination In
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Algorithmic Manipulation of Raw All
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Before You Begin Using SDS Online H
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Analyzing a Completed Allelic Discr
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Calling Allele Types To call allele
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Scrutinizing the Allele Calls To an
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After the Analysis Changing the Pla
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Real-Time Runs on the 7900HT Instru
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Overview About Absolute Quantificat
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Before You Begin Using SDS Online H
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Analyzing the Run Data Configuring
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Setting the Baseline and Threshold
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Eliminating Outliers For any PCR, e
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Viewing the Standard Curve 6-14 Rea
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6-16 Real-Time Analysis
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Overview About Dissociation Curve A
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Analysis Checklist WhereYouArein th
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Determining T m Values for the Anal
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After the Analysis Changing the Pla
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Recommended Maintenance Schedule Ma
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Replacing the Sample Block When to
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7-6 System Maintenance To remove th
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7-8 System Maintenance To replace t
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7-10 System Maintenance To replace
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Cleaning the Sample Block Wells 7-1
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Prepare a Background Plate Document
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Extracting the Background 7-16 Syst
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Materials Required The following ma
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Extracting Pure Dye Information fro
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Constructing a Custom Pure Dye Plat
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Verifying Instrument Performance Us
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Preparing and Running an RNase P Pl
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Adjusting the Sensitivity of the Pl
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Testing the Adjustment 7-30 System
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Aligning the Plate Handler When to
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7-34 System Maintenance To align th
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Re-checking the Input Stack 1 7-36
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Defining the Positions of the Remai
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Aligning the Fixed-Position Bar Cod
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7-42 System Maintenance To position
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7-44 System Maintenance
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General Computer Maintenance Mainte
Page 179 and 180: Maintaining the SDS software Admini
Page 181 and 182: Troubleshooting Table 8-2 Troublesh
Page 183 and 184: Low Precision or Irreproducibility
Page 185 and 186: Writing on the Reaction Plates Fluo
Page 187 and 188: Background Runs Background Troubles
Page 189 and 190: Pure Dye Runs Pure Dye Troubleshoot
Page 191 and 192: 8-12 Troubleshooting Troubleshootin
Page 193 and 194: Software and 7900HT Instrument Trou
Page 195 and 196: 8-16 Troubleshooting Troubleshootin
Page 198: User Bulletins 9 9 About This Chapt
Page 201 and 202: Fluorescent-Based Chemistries Funda
Page 203 and 204: Fluorescence Detection and Data Col
Page 205 and 206: Normalization of Reporter Signals A
Page 207 and 208: Determining Initial Template Concen
Page 209 and 210: Calculating Threshold Cycles A-10 T
Page 212 and 213: Importing and Exporting Plate Docum
Page 214 and 215: Configuring the Setup Table File wi
Page 216 and 217: About the Setup Table File Format S
Page 218 and 219: Setup Table Elements (continued) Nu
Page 220 and 221: Exporting Plate Document Data Expor
Page 222 and 223: Designing TaqMan Assays C In This A
Page 224 and 225: Design Probes and Primers The follo
Page 226 and 227: Design Tips for Allelic Discriminat
Page 228 and 229: Kits, Reagents and Consumables D In
Page 232: TaqMan Pre-Developed Assays and Rea
Page 236: Contacting Technical Support F Serv
Page 239 and 240: G-2 Limited Warranty Statement No a
Page 241 and 242: A(continued) Automation Controller
Page 243 and 244: I(continued) installing plate adapt
Page 245 and 246: Q quantifying probes and primers C-
Page 248 and 249: Headquarters 850 Lincoln Centre Dri
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