ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Troubleshooting Table
8-2 Troubleshooting
Overview The following table is designed to help you troubleshoot most of the problems you
may encounter while using the ABI PRISM® 7900HT Sequence Detection System.
The information in the table is arranged by category as follows:
♦ Chemistry problems
♦ Run problems
♦ Instrument and Automation Module Problems
Each category contains subcategories, followed by a brief description of the
symptoms you might encounter.
To use this table, look for the category and the symptom you are experiencing. The
page number in the right-hand column corresponds to a description of the possible
cause(s) and recommended action(s) for that particular problem.
Table 8-1 Troubleshooting Table
Category Symptom Page
Chemistry and Run Problems
Chemistry
Run Problems
Low Precision
Irreproducibility
8-4
Background Runs Software will not extract background data
Background is too high (greater than 2500)
8-8
Pure Dye Runs Software will not extract pure dye data
Raw data from pure dye run appears strange
Signals plateau (saturation)
Signal is too low (< 10,000 FSU)
More than two outliers per dye in a single row
8-10
Real-Time Runs (Quantitative PCR and Dissociation Curves) 8-11
End-Point Runs (Allelic Discrimination) 8-13