ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Getting Started
Before You Begin If this is your first time using the ABI PRISM ® 7900HT Sequence Detection System,
consider completing the “Basic Software Skills Tutorial” on page 3-11 before
continuing. The tutorial will provide you with the fundamental knowledge required to
operate the SDS software and will teach you time-saving techniques to allow you to
use it quickly and efficiently.
Procedure
Quick Reference
3-2 Getting Started
The following table contains a list of major procedures described within this manual.
Procedure See Page
Turning on the ABI PRISM 7900HT Sequence Detection System
Setting Up and Running SDS Experiments
3-5
Creating an SDS plate document 4-6
Running an individual SDS plate document 4-21
Running batches of SDS plate documents (using the automation accessory)
Stopping a run in progress
4-29
♦ From the SDS software 4-26
♦ From the Automation Controller Software
Ejecting a plate
4-38
♦ From the SDS software 4-27
♦ From the Automation Controller Software 4-38
Analyzing Run Data
Analyzing an allelic discrimination run 5-3
Analyzing an absolute quantification run 6-3
Analyzing a dissociation curve (melting curve) run
Maintaining the 7900HT Instrument
6-17
Changing the 7900HT Plate Adapter 7-9
Replacing the Sample Block 7-4
Decontaminating the Sample Block 7-11
Performing a Background Run 7-13
Performing a Pure Dye Run 7-17
Adding Custom Dyes to the Pure Dye Set 7-21
Verifying Instrument Performance Using a TaqMan RNase P Plate
Maintaining the Automation Accessory
7-24
a
Adjusting the Sensitivity of the Plate Sensor Switch 7-28
Aligning the Plate Handler 7-32
Aligning the Fixed-Position Bar Code Reader 7-40
Cleaning and Replacing Gripper Finger Pads 7-43
a. The automation accessory includes the Zymark® Twister Microplate Handler and the fixed-position bar
code reader. See “Instrument Components” on page 2-3 for more information.