ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Analyzing the Run Once you have configured the analysis options, you can analyze the run data. During
the analysis, the software mathematically transforms the raw data to establish a
comparative relationship between the spectral changes in the passive reference dye
and those of the reporter dye. Based on that comparison, the software calculates a
cycle threshold (C T ) for each reaction (standard and unknown). The software then
generates a standard curve for the run by plotting the standard samples on a graph of
C T versus initial copy number.
Note See Appendix A, “Theory of Operation,” for a detailed description of the SDS software
mathematical transformation of real-time run data.
To analyze the run:
Step Action
1 Select all wells in the plate grid.
The software outlines the selected wells with a black line.
2 Choose one of the following:
♦ From the Analysis menu, select Analyze.
♦ From the toolbar, click the Analyze button ( ).
The SDS software analyzes the run data and displays the results in the Results tab.
3 Set the baseline and threshold values for each detector on the plate as explained
on page 6-10.
Real-Time Analysis 6-9