ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Viewing Results
Viewing the
Analysis Table
Displays the results of the absolute quantification calculation in the results table of the
plate document. The figure below shows an example of the results table containing
the data from a TaqMan RNase P Instrument Verification Plate.
Table elements:
Column Displays
Position The coordinate position of the well on the plate.
Sample Name The sample name applied to the well.
Help button
Note Seepage4-19forinformationonapplyingsamplenamestothe
Detector
plate document.
The name of the detector assigned to the well.
Note See page 4-7 for information on applying detectors to the plate
Task
document.
The task (NTC, Standard, orUnknown) assigned to the well.
Note Seepage4-11forinformationonapplyingdetectortaskstothe
plate document.
CT The threshold cycle generated by the well during the PCR.
Quantity ♦ For wells containing Unknown samples, this column displays the
starting copy number for the well calculated by the software from the
standard curve.
♦ For wells containing Standard, this column displays the quantity
assigned to the well.
Note See page 4-11 for information on applying quantities to standard
wells of the plate document.
The following two columns contain data only if a well is run as part of a replicate group.
Qty Mean The arithmetic mean for the quantity values of the replicate group
associated with the well.
Qty stddev The standard deviation of the quantity values of the replicate group
associated with the well.
Real-Time Analysis 6-13