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ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

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7-8 <strong>System</strong> Maintenance<br />

To replace the sample block: (continued)<br />

Step Action<br />

7 If using a plate handler, replace the covers for the fixed-position bar code reader<br />

and the underlying platform (removed in step 6 on page 7-5).<br />

8 Plug in and turn on the <strong>7900HT</strong> instrument.<br />

9 Confirm the function of the installed sample block module as follows:<br />

a. Launch the Automation Controller Software.<br />

b. Click the Thermal Status tab.<br />

Does the software<br />

display temperatures? Then…<br />

Yes the installation is successful.<br />

10 Once the sample block is loaded into the instrument do the following:<br />

a. Perform a background run (see page 7-13) to verify that the sample block:<br />

– Is connected and working properly<br />

– Contains no contaminants that will interfere with fluorescent detection<br />

b. If changing sample block formats, perform any remaining tasks outlined in the<br />

“Sample Block Installation Checklist” on page 7-4.<br />

GR2009<br />

Fixed-position bar code reader<br />

and underlying platform covers<br />

The presence of temperature readings confirm that<br />

the <strong>7900HT</strong> instrument successfully established the<br />

connection to the new sample block.<br />

No the <strong>7900HT</strong> instrument is unable to establish<br />

communication with the new sample block.<br />

To troubleshoot the problem:<br />

a. Turn off and unplug the <strong>7900HT</strong> instrument.<br />

b. Remove the thermal cycler access cover.<br />

c. Press on the right and left sides of the front plate<br />

of the sample block to ensure that it is seated<br />

securely.<br />

d. Reinstall the thermal cycler access cover.<br />

e. Repeat step 8 until you hear a high-pitched tone<br />

confirming communication between the<br />

instrument and sample block.

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