ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

P
panes
hiding 3-16
maximizing/minimizing 3-15
resizing 3-15
showing 3-16
passive reference
setting 4-12
use in multicomponent analysis A-5
PCR
5´ Nuclease Assay A-2
Geometric (Exponential) Phase A-7
kinetic analysis of A-7
Linear Phase A-8
Plateau Phase A-8
SYBR Green Chemistry A-3
pipetting errors 8-5
pipettors, using 8-5
plate adapter, changing 7-9
plate document information
applying to a plate document 4-19
plate documents 4-6
about 3-11
adding to the plate queue 4-31, 4-35
applying detector tasks 4-11
applying sample names 4-19
assigning standard quantities 4-12
closing 3-14
configuring document information 4-19
copying detectors 4-8
copying markers 4-10
creating 3-12, 4-6
from a template 4-18, 4-32
exporting 3-13
importing setup data 3-14, B-2
methods, See methods
opening 3-13
programming methods 4-13
removing from the plate queue 4-35
running
in batches 4-34
individually 4-20
saving 3-12
as a single plate file 4-22
as a template file 4-17
setting Sample Volume 4-15
setting the passive reference 4-12
plate grid
about 3-21
eliminating wells from use 6-12
selecting wells 3-17
viewing well information 3-16
zooming 3-18
plate handler 2-9, 7-27
aligning 7-32 to 7-39
arm
adjustment knob 7-27
gripper 7-27
plate-sensor switch 7-27
cleaning the finger pads 7-43
plate stack positions 7-27
replacing the finger pads 7-43
turning ON 3-5
plate queue
adding plate documents
from the Automation Controller software 4-35
from the SDS software 4-31
using the Template Batch utility 4-32
removing plate documents 4-35
starting 4-38
stopping 4-38
plate stacks
loading plates 4-36, 4-37
placing in use 4-37
positions 7-27
Plateau Phase of the amplification curve A-8
plates
running
batches 4-34
individually 4-23
types, See consumables
plate-sensor switch 7-27
adjusting 7-28 to 7-31
plots
hiding 3-16
maximizing/minimizing 3-15
resizing 3-15
showing 3-16
Post readout (from the Plate Read tab) 4-25
precision
causes of low precision 8-4 to 8-7
preparing
master mixes C-4
plate for a run 4-23
primer and probe concentrations
optimizing C-4
programming
methods
for absolute quantification 4-14
for allelic discrimination 4-14
for dissociation curve analysis 4-16
temperature ramp 4-16
pure dye plate
constructing for custom dyes 7-21
preparing for use 7-19
pure dye run
about 7-17
creating a plate document 7-18
extracting data 7-20
performing 7-19
troubleshooting 8-10
when to perform 7-17
Index-5