ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Exporting Plate Document Data
ExportingDatafrom
aPlateDocument
TheSDSsoftwarecanexportraworanalyzeddataintab-delimited(*.txt)formatforall
or a select group of wells on a plate document. The exported files are compatible with
most spreadsheet applications.
To export an element of a plate document:
Step Action
1 Click the plate document to select it.
Note The plate document must be the top-most object in the workspace.
2 From the File menu, select Export.
3 Navigate to the directory you would like to receive the exported file(s).
4 Click the Export drop-down list, and select the type of data you would like the
software to export.
Data Type The exported file contains…
Background
Spectra
Fluorescence readings for each well from the background component
used to analyze the run
Clipped Average R n and ∆R n of the last three data points collected during the
extension phase of each cycle repetition for each well
Dissociation
Curve
♦ Temperature Data – For each well in use on the plate, the file
displays the calculated temperature of the wells during each data
collection reading of the temperature ramp.
♦ Raw Data – For each well in use on the plate, the file displays the
Rn of the well during each data collection reading of the
temperature ramp.
♦ Derivative Data – Foreachwellinuseontheplate,thefiledisplays
the first derivative data during each data collection reading of the
temperature ramp.
Multicomponent ♦ Calculated amounts the dye components in a single well throughout
all stages of the PCR that were labeled with data collection icons
♦ Pure spectra component data
♦ Calculated inverse matrix
♦ Singular values of the inverse matrix
Pure Spectra Fluorescence readings for each well from the pure spectra calibration
component used to analyze the run
Raw Spectra Unmodified fluorescence readings taken for each spectral bin during
the course of the run
When exported, the software creates a directory and saves each the
raw spectra data for each well in a separate *.txt file.
Results Table The contents of the results table of an analyzed plate document
Note The contents of the exported data varies depending on the
Setup Table
type of plate document.
The contents of the results table of a plate document prior to analysis
The contents of the exported data varies depending on the type of
plate document used to produce it. See page B-4 for a detailed
description of the Setup Table file.
Importing and Exporting Plate Document Data B-9