ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare
ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare
ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare
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Section:DissociationCurveAnalysis....................................6-17<br />
Overview............................................................6-18<br />
BeforeYouBegin .....................................................6-19<br />
AnalysisChecklist.....................................................6-20<br />
AnalyzingtheRunData ................................................6-21<br />
Determining Tm ValuesfortheAnalyzedRun ...............................6-22<br />
AftertheAnalysis.....................................................6-24<br />
7 <strong>System</strong> Maintenance<br />
RecommendedMaintenanceSchedule......................................7-2<br />
Section: Maintaining the <strong>7900HT</strong> Instrument .............................7-3<br />
ReplacingtheSampleBlock..............................................7-4<br />
Changingthe<strong>7900HT</strong>PlateAdapter........................................7-9<br />
DecontaminatingtheSampleBlock .......................................7-11<br />
PerformingaBackgroundRun ...........................................7-13<br />
PerformingaPureDyeRun..............................................7-17<br />
AddingCustomDyestothePureDyeSet ..................................7-21<br />
Verifying Instrument Performance Using a TaqMan RNase P Plate. . . . . . . . . . . . . . . 7-24<br />
Section:MaintainingthePlateHandler .................................7-27<br />
Adjusting the Sensitivity of the Plate Sensor Switch . . . . . . . . . . . . . . . . . . . . . . . . . . 7-28<br />
AligningthePlateHandler ..............................................7-32<br />
Aligning the Fixed-Position Bar Code Reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-40<br />
CleaningandReplacingGripperFingerPads................................7-43<br />
Section:MaintainingtheComputerandSDSSoftware .....................7-45<br />
GeneralComputerMaintenance..........................................7-46<br />
MaintainingtheSDSsoftware............................................7-48<br />
8 Troubleshooting<br />
TroubleshootingTable...................................................8-2<br />
Low Precision or Irreproducibility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-4<br />
BackgroundRuns ......................................................8-8<br />
PureDyeRuns........................................................8-10<br />
Real-TimeRuns(QuantitativePCRandDissociationCurves)...................8-11<br />
End-PointRuns(AllelicDiscrimination) ...................................8-13<br />
Softwareand<strong>7900HT</strong>Instrument.........................................8-14<br />
Zymark Twister Microplate Handler and Fixed-Position Bar Code Reader . . . . . . . . . 8-17<br />
9 User Bulletins<br />
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