ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

End-Point Runs on the 7900HT Instrument
End-Point Runs End-point is the term used to describe the category of sequence detection runs in
which the ABI PRISM ® 7900HT Sequence Detection System is used to measure the
fluorescence of a biological sample after it has undergone thermal cycling. Unlike
real-time runs that can yield quantitative measurements, the focus of end-point
experiments is typically to produce a qualitative result. End-point analysis is
commonly used in combination with TaqMan ® chemistry to confirm the presence or
absence of specific target nucleic acid sequence in cells, tissues, or fluid samples.
5-2 End-Point Analysis
Currently, the SDS software supports one type of end-point analysis: Allelic
Discrimination.