ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

To create a template for running the custom pure dye plate: (continued)
Step Action
4 Apply pure dyes to the custom plate document as follows:
a. Select the wells containing the custom dye.
b. From the Setup tabbed page, click the Dyes drop-down list, and select the
appropriate dye.
The software applies the dye to the selected wells.
c. Repeat steps 6 and 7 to configure the plate document with any additional
custom dyes.
Dyes drop-down list
Custom dye added to selected
wells of the plate document
5 Save the custom pure dye plate document as a template file as follows:
a. From the File menu, select Save.
The Save dialog appears.
b. Navigate to Program Files > Applied Biosystems > SDS 2.0 > Templates.
The Templates directory appears within the Look in text field. By saving the
template to the Templates folder, it becomes available from the Template
drop-down list in the New Document dialog box.
c. From the Files of type drop-down list, select ABI PRISM SDS Template Document.
d. Click the File name text field, and type a name for the template document.
e. Click Save.
The software saves the plate document as a template file (*.sdt).
6 Run the custom pure dye plate as explained in “Preparing and Running the
Pure Dye Plate” on page 7-19.
System Maintenance 7-23