ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Analyzing the Run Data
Analyzing the Run The run data from a temperature ramp can be analyzed immediately following the
completion of the run. For an explanation of how the software manipulates the raw
data, see “Algorithmic Manipulation of Raw Data” on page 6-5.
To analyze the run:
Step Action
1 If not already open, launch the SDS software as explained on page 4-5.
2 Open the plate document file for analysis as follows:
a. From the File menu, select Open.
b. From the File Type drop-down list, select ABI PRISM SDS Single Plate (*.sds).
c. From the Look in text field, navigate to and select the plate document file.
d. Click Open.
The SDS software displays the plate document file.
3 Select all wells in the plate grid.
The software outlines the selected wells with a black line.
4 Choose one of the following:
♦ From the Analysis menu, select Analyze.
♦ From the toolbar, click the Analyze button ( ).
The software analyzes the run data and displays the results in the Dissociation
Curve tab.
5 Determine the Tm for the dissociation curves displayed within the Dissociation
Curve tabbed page as explained on page 6-23.
Real-Time Analysis 6-21