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ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

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Tabbed Pages Plate documents can contain up to six tabbed pages depending on their function:<br />

Well Inspector The Well Inspector is used to apply detector and sample information to the wells<br />

within the grid pane and to display information from the selected cells in the plate grid.<br />

3-22 Getting Started<br />

Tab Used to...<br />

Setup display well information, and to configure the plate grid with setup information.<br />

Instrument program the plate document method, and to run the plate document or send<br />

it to the Plate Queue.<br />

Raw Data display the raw fluorescence collected from the sequence detection run.<br />

Calibration display the Background and Pure Spectra calibration data used for the signal<br />

Data normalization and multicomponenting analysis of the current run.<br />

Results display analyzed run data. The Analysis tab is visible only in plate documents<br />

containing analyzed run data.<br />

Dissociation display analyzed dissociation curve data from a programmed ramp. The<br />

Curve Dissociation Curve tab is visible only in plate documents containing analyzed<br />

data from a real-time run with a programmed ramp.<br />

Component Description<br />

Sample name<br />

text field<br />

An editable text field that displays the sample name applied to the<br />

selected well(s)<br />

Note The Sample name text field will display *Mixed* if multiple<br />

wells with different sample names are selected.<br />

Detector list Lists all available detectors copied to the plate document<br />

In Use check box Toggles the activity of the well. If unchecked, the software eliminates<br />

the data from the selected well from all analysis procedures.<br />

Passive Reference<br />

drop-down list<br />

Sample name text field<br />

Detector list<br />

Passive Reference drop-down list<br />

In Use check box<br />

Displays the fluorescent dye used as a passive reference<br />

Marker Inspector<br />

Used only for allelic discrimination runs, the Marker Inspector (shown below) appears<br />

as the lower half of the Well Inspector and displays all markers available for the plate.<br />

Marker inspector

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