ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Creating an Allelic
Discrimination
Marker
Allelic discrimination plate documents feature the use of ‘markers’ to aid in organizing
and applying detectors based on the loci they target. A marker is a pairing of two
detectors representing chemical assays designed to amplify different alleles of a
common locus. The SDS software uses marker information during data analysis to
organize and compare the processed run data.
IMPORTANT Allelic discrimination plate documents must contain at least one marker.
To create a marker for an allelic discrimination plate document:
Step Action
1 If the Detector Manager dialog box is open, click Done to close it.
2 From the Tools menu of the SDS software, select Marker Manager.
The Marker Manager dialog box appears.
3 Click Create Marker.
The Add Marker dialog box appears.
4 Click the Enter name of new Marker text field, type a name for the new marker, and
click OK.
The new marker appears within the Markers text field.
5 Apply detectors to the new marker and follows:
a. From the Markers text field, click the new marker to select it.
The software highlights the selected marker.
b. From the Available Detectors text field, click a detector that you want to add to
the marker.
The software highlights the selected detector.
c. Click Add Detector.
The software applies the detector to the marker and displays it below the Marker
entry.
d. Repeat steps b and c for the remaining detector.
Detectors assigned to
Marker ‘CYP 2C9*2’
6 If evaluating multiple loci, repeat steps 3-5 to create additional markers as needed.
IMPORTANT A marker must be configured with two detectors before it can be
applied to a plate document.
Note Click the button for information on the features of the Marker Manager
7
dialog box or to view the procedure for deleting markers from the Markers list.
Apply the marker(s) to the allelic discrimination plate document as explained in
“Copying and Applying Markers to a Plate Document” on page 4-10.
Run Setup and Basic Operation 4-9