ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Materials Required The following materials are required to perform a pure dye run:
Preparing a
Pure Dye Plate
Document
7-18 System Maintenance
Material
Sequence Detection Systems Spectral Calibration Kit
Part Number
384-Well Version
96-Well Version
See page D-3
Pure Dye Platea,b —
Product Insertb —
Centrifuge, with plate adapter —
a. The 96-Well version of the Spectral Calibration Kit contains 2 Pure Dye plates.
b. Included with the Sequence Detection Systems Spectral Calibration Kit.
IMPORTANT A background run must be performed prior to running a pure dye plate. See
“Performing a Background Run” on page 7-13 for more information.
To prepare a plate document for the pure dye run:
Step Action
1 Remove the pure dye plate from the freezer, place it aside to thaw to room
temperature, and return to the computer.
2 Launch the SDS software.
3 From the File menu, select New.
The New Document dialog appears.
4 Select the following options from the menus within the New Document dialog box.
From… Select…
Assay Pure Dyes
Container
Template ♦ For a 384-Well Pure Dye Run, select
384 Well Pure Dyes Plate.sdt.
♦ For 96-Well Pure Dye Runs, select the template matching the
Pure Dye plate you intend to run.
– To run Plate 1 (containing FAM, JOE, NED, and ROX), select
96 Well Pure Dyes Plate 1.sdt.
– To run Plate 2 (containing SYBR, TAMRA, TET, and VIC),
select 96 Well Pure Dyes Plate 2.sdt.
Note If no templates are available, construct a Pure Dye plate
document using the product insert from the Sequence Detection
Systems Spectral Calibration Kit and the procedure on page 7-22.
5 Remove the pure dye plate from its packaging.
6 Click the Barcode text field, and scan the bar code number using the hand-held bar
code reader.
7 Click OK.
The software displays a plate document with the attributes for a pure dye run.