ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Significance of
Threshold Cycles
Beginning with the equation describing the exponential amplification of the PCR:
Xn = Xm( 1+ EX) where:
n m –
Xn = number of target molecules at cycle n (so that n ≥ m)
Xm = number of target molecules at cycle m (so that m ″ n)
EX = efficiency of target amplification (between 0–1)
n - m = number of cycles elapsed between cycle m and cycle n
Amplicons designed and optimized according to Applied Biosystems guidelines
(amplicon size