ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Analysis Checklist
WhereYouArein
the Procedure
5-8 End-Point Analysis
The following checklist illustrates your current position in the overall procedure:
Done Step Procedure See Page
✓ 1 Create an allelic discrimination plate document. 4-6
✓ 2a a. Create detectors for the allelic discrimination probes. 4-7
b. Create a marker for each allelic discrimination probe
pairing.
4-9
c. Copy the marker(s) to the plate document. 4-10
✓ 3a Assign detector tasks to the wells of the plate document
(NTC and Unknown).
4-11
✓ 4 If you would like to perform thermal cycling of the allelic
discrimination plate on the 7900HT instrument, create a
real-time plate document for the plate and program it with
the method for the allelic discrimination run. Otherwise,
continue to step 5.
✓ 5 Choose from the following:
✓ 6 Create a plate document from the template created in
step 5.
✓ 7 Configure the document with sample names and plate
information.
a. Steps 2 and 3 can be eliminated by importing the plate document setup information from a tab-delimited
text file. See “Importing Plate Document Setup Table Files” on page B-2 for more information.
4-13
If running… Then…
a single plate continue to step 7.
the first plate in a series Save the plate document as an ABI PRISM
of plates with identical SDS Template Document as explained on
assay configurations page 4-17.
4-18
4-19
✓ 8 a. Prepare the allelic discrimination plate or plates and
perform thermal cycling on a designated thermal cycler.
b. Run the allelic discrimination plate or plates on the
7900HT instrument.
4-20
9 Analyze the run data. 5-9
10 View the results of the allelic discrimination run. 5-10
11 Call allele types for each marker. 5-11
12 Scrutinize the allele calls. 5-13
13 Choose from the following post-analysis options:
♦ Reanalyze the run data.
♦ Adjust the display settings for the plate document.
♦ Print elements of the plate document.
♦ Export the plate document results table or plots.
5-15