ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Loading Plates onto the Automation Module
Pre-Run Checklist The following tasks must be complete to run plates on the 7900HT instrument. See
the associated page number for details on each procedure.
Plate Requirements See “Consumables and Disposables” on page D-3 for a complete list of ABI PRISM
consumables and ordering instructions.
Guidelines Observe the following guidelines when loading plates onto the plate handler:
4-36 Run Setup and Basic Operation
Done Check See Page
A background run has been performed in the last month 7-13
A pure dye run has been performed in the 6 months 7-17
The plate queue contains plate documents for all plates to be run 4-6
The instrument tray does not contain a plate
IMPORTANT The instrument tray must be empty to begin a run. If
the instrument tray contains a plate, eject and remove it before
continuing.
The output stack does not contain plates. –
♦ Before loading plates onto the plate handler, make sure that for each plate:
– the associated plate document has been added to the plate queue
– the plate has been sealed using an optical adhesive cover.
♦ Load the plates into the plate handler stacks in any order. The software reads the
bar code of each plate before it is run and matches the plate document and
method with the plate. The Automation Controller Software can run batches of up
to 84 plates in a single session (21 plates/stack).
♦ Orient the plates within the stacks so that well A1 ( ) of each plate corresponds to
the locations shown in the illustration below.
♦ Do not place plates in the output stack (X). The Zymark arm uses the empty stack
to store used plates after they are run.
Zymark Twister Microplate Handler
(top view)
X
1
4
2
3
Bar code
Well A1
4-38