ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Cleaning the Sample
Block Wells
7-12 System Maintenance
To clean the sample block wells:
Step Action
1 Identify the contaminated wells as follows:
a. If not already run, perform a background run as explained on page 7-13.
b. Identify the contaminated wells on the sample block by following the procedure,
“Isolating Sample Block Contamination” on page 8-8.
2 Remove the sample block from the 7900HT instrument as explained in “Removing
the Sample Block” on page 7-5.
3 Using the following figure as a guide, locate the suspected contaminated wells on
the sample block.
Warning
GR2028
Circuitry and connections to
the instrument (Do Not Touch)
Well A-1
4 Pipet the appropriate volume of 10% bleach solution into each suspected
contaminated well of the sample block module.
♦ For a 96-well sample block, pipet 150 µL bleach solution to each well.
♦ For a 384-well sample block, pipet 40 µL bleach solution to each well.
5 Allow the sample block to sit for 3-5 min.
6 Using a pipet, remove the bleach solution from the wells of the sample block.
7 Rinse (pipet and remove) each contaminated well with 3 treatments of deionized
water at the appropriate volume for the sample block.
♦ For a 96-well sample block, rinse affected wells with 150 µL deionized water.
♦ For a 384-well sample block, rinse affected wells with 40 µL deionized water.
Note Absolute isopropanol can be substituted for water in the third treatment.
8 Remove any remaining isopropanol or water from the wells of the sample block
module.
9 Replace the sample block as explained in “Replacing the Sample Block” on
page 7-7.
10 Run a background plate to confirm that the contamination has been removed.