ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

D
DANGER attention word, definition 1-2
data collection A-5
data type definitions (exportable) B-9
decontaminating the sample block module 7-11 to 7-12
defragmenting the hard drive 7-47
deleting, steps from a method 4-15
detector tasks
about 4-11
applying 4-11
importing into a plate document B-2
detectors
about 4-7
applying to a plate document 4-8
copying to a plate document 4-8
creating 4-7
importing into a plate document B-2
tasks, See detector tasks
determining melting temperatures 6-23
disconnecting the SDS software 4-28
display settings, adjusting 4-17
dissociation curve
about 6-18
analyzing 6-20 to 6-24
definitions of the T m value 6-22
procedure checklist 6-20
programming a temperature ramp 4-16
setting up (procedure checklist) 4-4
Dissociation Plot
about 6-22
exporting as a graphic file B-8
exporting data as a text file B-9
E
ejecting a plate from the 7900HT instrument 4-27, 4-38
eliminating
outliers 6-12
wells from use 6-12
emission filter
See instrument, optics system
end-point runs
about 5-2
allelic discrimination, See allelic discrimination
entering
bar code information 3-19, 4-19
using the Template Batch utility 4-32
comments
into a detector 4-7
into a plate document 4-19
sample names into a plate document 4-19
exportable data type definitions B-9
exporting
data from a plate document 3-13, B-9
plotsandviewsasgraphicfiles B-8
F
finger pads
cleaning 7-43
replacing 7-43
fixed-position bar code reader
aligning 7-40 to 7-42
connections 2-11
location 2-8
specification 2-8
fluorescent
contamination 8-6
detection system 2-11, A-4
fluorogenic probe
about A-2
designing C-2 to C-3
G
genotypic segregation of datapoints
(Allelic Discrimination Plot) 5-6
Geometric (Exponential) Phase A-7
grating, See instrument, optics system
grey dividing line, using 3-15
grid, See plate grid
gripper 7-27
guidelines
assay development
absolute quantification C-6
allelic discrimination C-5
loading the plate handler 4-36
setting the baseline 6-10
setting the threshold 6-11
TaqMan probe design C-2 to C-3
H
hand-held bar code reader
connections 2-11
location 2-8
specification 2-8
using 3-19
hard drive
defragmenting 7-47
partitions 2-7
heated clamp 2-4
help
background information 3-3
using the SDS Online Help 3-3
hold, adding to a method 4-15
hotkey combinations, using 3-20
I
Important attention word, definition 1-2
importing setup table data 3-14, B-2
imprecise pipetting 8-5
improper threshold setting 8-4
Index-3