ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Before You Begin
Using SDS
Online Help
Examples in This
Chapter
6-6 Real-Time Analysis
For specific instructions on any procedure described within this section, refer to the
online help accompanying the SDS software. To get help at any time during the
procedure, click a help button ( ) located within the dialog box or window in which
you are working.
The illustrations and screenshots that appear within this chapter were created for a
TaqMan® RNase P Instrument Verification Plate, an experiment run during the
installation of the 7900HT instrument to verify its performance. The sealed plate is
pre-loaded with the reagents necessary for the detection and quantification of
genomic copies of the human RNase P gene (a single-copy gene encoding the moiety
of the RNase P enzyme). Each well contains pre-loaded reaction mix (1X TaqMan®
Universal PCR Master Mix, RNase P primers, and FAM-labeled probe) and
template.
The following figure illustrates the arrangement of standards and samples on the
RNase P plate. As shown below, the RNase P plate consists of 5 columns of template
standards (1250, 2500, 5000, 10,000, and 20,000 copies) and two unknown
populations (5000 and 10,000 copies).
Unknown 1
5000
NTC
STD 1250
STD 2500
STD 5000
STD 10000
STD 20000
Unknown 2
10000
GR2107