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ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

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Defining the Bottom<br />

of the Stack<br />

To re-check the position of input stack 1 (Zymark position 4): (continued)<br />

Step Action<br />

8 Click Rotary Offset, andclickYes.<br />

The software re-records the rotary position for the input stack 1(Zymark position 4).<br />

9 While holding the plate, click Open Gripper and remove the plate.<br />

The Automation Controller Software requires a bottom position value for all stacks.<br />

This value is used to prevent the plate handler arm from colliding or grinding as it<br />

moves to the bottom of each stack.<br />

To find the bottom of the stack:<br />

Step Action<br />

1 Remove all plates from the instrument and the plate handler arm.<br />

2 Place an empty plate into the output stack (Zymark position 0).<br />

3 From the Zymark Twister Software, click position 0.<br />

The plate handler arm moves over the output stack.<br />

4 Using the Vertical Positioning commands, lower the plate handler arm until it is just<br />

above the stack.<br />

5 Check the rotary position of the plate handler arm to confirm that the gripper:<br />

♦ is centered over the stack<br />

♦ will not contact the sides of the stack when lowered<br />

6 Using the Rotary Adjustment arrows, adjust the rotational position of the gripper so<br />

that it is centered over the input stack and will not contact the sides when lowered.<br />

7 Using the Vertical Positioning commands, carefully lower the plate handler arm into<br />

the stack. Adjust the Rotary Adjustment value as needed to center the gripper<br />

within the stack.<br />

8 Once the gripper is centered within the stack, click Find Plate.<br />

The plate handler arm lowers upon the plate.<br />

Confirm the following:<br />

♦ The plate is in the middle of the gripper span<br />

♦ The plate sensor switch is contacting the plate<br />

♦ The gripper does not contact the side of the stack<br />

9 Click Close Gripper.<br />

The gripper grips the plate between its fingers.<br />

10 Select Vertical Home.<br />

The plate handler raises the arm to its highest position. If the plate contacts the<br />

sides of the stack, re-adjust the rotary position of the plate handler arm until the<br />

plate moves freely in the stack.<br />

Note Contact between the plate and the stack or all stacks may be unavoidable.<br />

However, try to minimize the contact as much as possible.<br />

11 Using the Vertical Positioning commands, raise and lower plate handler arm several<br />

times to check the alignment.<br />

12 Lower the plate handler arm and click Rotary Offset, and click Yes.<br />

The software records the rotary position for position 0 (the output stack).<br />

<strong>System</strong> Maintenance 7-37

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