ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Working with SDS Software Files
Files Used and
Created by the
SDS Software
Average SDS Plate
Document File Size
2-16 Product Overview
IMPORTANT Never move or delete a SDS software file or folder unless specifically directed to
do so by an Applied Biosystems representative or documentation.
The SDS software includes many different files and folders. Some of these are
created to store run data and calibration data, others are required to run the software.
File Type Extension Description
Plate Document Files
ABI PRISMSDS *.sds A plate document is a virtual representation of a
Single Plate
consumable (plate) used to contain samples and reagents
during an sequence detection run. During the run, the
software uses the plate document to coordinate the
operation of the instrument (thermal cycling, data
collection), to organize and store the data gathered during
the PCR, and to store the results of the downstream
analysis of the run data.
ABI PRISMSDS *.sdt Templates contain can be used to create an unlimited
Template File
number of plate documents. Templates are optional but
useful as a time-saving devices for experiments where
samples are run on plates with identical assay
configurations.
Imported/Exported Files
Tab-delimited *.txt The SDS software can export raw or analyzed data in
text file
tab-delimited (*.txt) format for all or a select group of wells
on a plate document. The exported files are compatible with
most spreadsheet applications.
JPEGa graphic *.jpg The SDS software can export most panes and plots of the
files
plate document as JPEG graphic files. The JPEG format is
compatible with most word processing and spreadsheet
applications and can be incorporated directly into HTML
documents for viewing by most web browser software.
a. Joint Photographic Experts Group
The SDS software can produce SDS files of varying sizes depending on the type of
runs for which they are created. The table below lists the average sizes of typical files
produced by the SDS software.
Run Type Average File Size Compressed File Size a
Plate-read 150–180 KB 70–90 KB
Real-time b
15–25 MB 10–15 MB
a. Compressed files sizes shown are estimates based on standard compression using the WinZip® utility.
For more information, see “Archiving SDS Files” on page 7-46.
b. The maximum file sizes displayed above are nominal for real-time runs (absolute quantification). File size
can increase depending on the plate document’s data collection options.