ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Setup Table Elements (continued)
Number Contents Description
Detector Definitions Element numbers 4 to 6 define the detectors that will appear in the well descriptions that
follow in a later section. The detector definition consists of three sections: the declaration of
the number of detectors, the detector list header, and the detector list.
4 Number of
Detectors
Format:
This line defines the total number of detectors on the plate.
[ *** Number of Detectors number of detectors ]
Example:
*** Number of Detectors 5
5 Detectors
List Header
Format:
B-6 Importing and Exporting Plate Document Data
This line contains the column headings for the Detector Definitions section of the setup table
file that make the file easier to edit using a program such as Microsoft Excel.
[ Detector Reporter Quencher Description Comments
Sequence ]
Example:
Detector ReporterQuencher Description Comments Sequence
6 Detectors
List
Format for a single detector:
The detector list consists of one or more lines displaying the information for each detector
used on the plate document.
♦ The Detectors List section must contain one line (or definition) for each detector present
on the plate.
♦ The number of lines in the Detectors List section must be equal to the number defined in
the Number of Detectors section (see number 4 above).
♦ Leave blank the Quencher Dye entry for detectors created for the SYBR Green I Dye or
probes labeled with a non-fluorescent quencher.
♦ The Sequence text value contains the name of the Allelic Discrimination marker
associated with the detector. Because markers are used exclusively in allelic
discrimination plate documents, assign Sequence text values to detector definitions of
allelic discrimination setup table files only.
[ detector name reporter dye quencher dye description
comments sequence ]
Example for an allelic discrimination setup table file:
CYP 2C9*2.1 FAM PDAR CYP 2C9*2 Allele 1Example ProbeCYP 2C9*2
CYP 2C9*2.2 VIC PDAR CYP 2C9*2 Allele 2Example ProbeCYP 2C9*2
Example for an absolute quantification setup table file:
GAPDH VIC GAPDH Probe Example Probe
SYBR Green SYBR SYBR Green I Example Probe
RNase P FAM TAMRA RNase P Probe Example Probe