- Page 1 and 2: ABI PRISM ® 7900HT Sequence Detect
- Page 3 and 4: © Copyright 2001, Applied Biosyste
- Page 5 and 6: iv Section:PlateDocumentSetup......
- Page 7 and 8: vi A Theory of Operation Fluorescen
- Page 9 and 10: Attention Words and Warning Labels
- Page 11 and 12: About Waste Profiles A waste profil
- Page 13 and 14: Safe Instrument Use Before Operatin
- Page 16 and 17: Product Overview 2 In This Chapter
- Page 18 and 19: Section: Getting to Know the Hardwa
- Page 20 and 21: Interchangeable Thermal Cycler Bloc
- Page 22 and 23: Computer Description The computer c
- Page 26 and 27: Instrument Connections Electrical C
- Page 28 and 29: Section: Getting to Know the Softwa
- Page 30 and 31: Managing Sequence Detection System
- Page 32 and 33: Getting Started 3 In This Chapter T
- Page 34 and 35: Using the SDS Software Online Help
- Page 36 and 37: Turning on the ABI PRISM 7900HT Seq
- Page 38 and 39: Using the SDS Software Workspace La
- Page 40 and 41: Using the General Toolbar Using the
- Page 42 and 43: Basic Software Skills Tutorial Abou
- Page 44 and 45: Exercise 3: Opening a Plate Documen
- Page 46 and 47: Lesson 2: Viewing and Resizing Pane
- Page 48 and 49: Exercise 2: Selecting Multiple Well
- Page 50 and 51: Lesson 4: Using the Hand-Held Bar C
- Page 52 and 53: Using SDS Plate Documents Using Mul
- Page 54 and 55: Run Setup and Basic Operation 4 In
- Page 56 and 57: Setup Checklists Experiments/Runs P
- Page 58 and 59: Section: Plate Document Setup In Th
- Page 60 and 61: Step 2 - Applying Detectors and Mar
- Page 62 and 63: Creating an Allelic Discrimination
- Page 64 and 65: Step 3 - Configuring the Plate Docu
- Page 66 and 67: Step 4 - Programming the Plate Docu
- Page 68 and 69: To create a method for the absolute
- Page 70 and 71: Step 5 - Saving the Plate Document
- Page 72 and 73: Step 7 - Applying Sample and Plate
- Page 74 and 75:
Section: Running an Individual Plat
- Page 76 and 77:
Preparing and Running a Single Plat
- Page 78 and 79:
Operating the 7900HT Instrument Usi
- Page 80 and 81:
After the Run Analyzing the Run Dat
- Page 82 and 83:
Section: Running Multiple Plates Us
- Page 84 and 85:
Adding a Plate Document to the Plat
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To create plate documents from a te
- Page 88 and 89:
Adding Plates to the Plate Queue Re
- Page 90 and 91:
Loading Plates To load plates onto
- Page 92 and 93:
End-Point Analysis 5 In This Chapte
- Page 94 and 95:
Section: Allelic Discrimination In
- Page 96 and 97:
Algorithmic Manipulation of Raw All
- Page 98 and 99:
Before You Begin Using SDS Online H
- Page 100 and 101:
Analyzing a Completed Allelic Discr
- Page 102 and 103:
Calling Allele Types To call allele
- Page 104 and 105:
Scrutinizing the Allele Calls To an
- Page 106:
After the Analysis Changing the Pla
- Page 109 and 110:
Real-Time Runs on the 7900HT Instru
- Page 111 and 112:
Overview About Absolute Quantificat
- Page 113 and 114:
Before You Begin Using SDS Online H
- Page 115 and 116:
Analyzing the Run Data Configuring
- Page 117 and 118:
Setting the Baseline and Threshold
- Page 119 and 120:
Eliminating Outliers For any PCR, e
- Page 121 and 122:
Viewing the Standard Curve 6-14 Rea
- Page 123 and 124:
6-16 Real-Time Analysis
- Page 125 and 126:
Overview About Dissociation Curve A
- Page 127 and 128:
Analysis Checklist WhereYouArein th
- Page 129 and 130:
Determining T m Values for the Anal
- Page 131 and 132:
After the Analysis Changing the Pla
- Page 133 and 134:
Recommended Maintenance Schedule Ma
- Page 135 and 136:
Replacing the Sample Block When to
- Page 137 and 138:
7-6 System Maintenance To remove th
- Page 139 and 140:
7-8 System Maintenance To replace t
- Page 141 and 142:
7-10 System Maintenance To replace
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Cleaning the Sample Block Wells 7-1
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Prepare a Background Plate Document
- Page 147 and 148:
Extracting the Background 7-16 Syst
- Page 149 and 150:
Materials Required The following ma
- Page 151 and 152:
Extracting Pure Dye Information fro
- Page 153 and 154:
Constructing a Custom Pure Dye Plat
- Page 155 and 156:
Verifying Instrument Performance Us
- Page 157 and 158:
Preparing and Running an RNase P Pl
- Page 159 and 160:
Adjusting the Sensitivity of the Pl
- Page 161 and 162:
Testing the Adjustment 7-30 System
- Page 163 and 164:
Aligning the Plate Handler When to
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7-34 System Maintenance To align th
- Page 167 and 168:
Re-checking the Input Stack 1 7-36
- Page 169 and 170:
Defining the Positions of the Remai
- Page 171 and 172:
Aligning the Fixed-Position Bar Cod
- Page 173 and 174:
7-42 System Maintenance To position
- Page 175 and 176:
7-44 System Maintenance
- Page 177 and 178:
General Computer Maintenance Mainte
- Page 179 and 180:
Maintaining the SDS software Admini
- Page 181 and 182:
Troubleshooting Table 8-2 Troublesh
- Page 183 and 184:
Low Precision or Irreproducibility
- Page 185 and 186:
Writing on the Reaction Plates Fluo
- Page 187 and 188:
Background Runs Background Troubles
- Page 189 and 190:
Pure Dye Runs Pure Dye Troubleshoot
- Page 191 and 192:
8-12 Troubleshooting Troubleshootin
- Page 193 and 194:
Software and 7900HT Instrument Trou
- Page 195 and 196:
8-16 Troubleshooting Troubleshootin
- Page 198:
User Bulletins 9 9 About This Chapt
- Page 201 and 202:
Fluorescent-Based Chemistries Funda
- Page 203 and 204:
Fluorescence Detection and Data Col
- Page 205 and 206:
Normalization of Reporter Signals A
- Page 207 and 208:
Determining Initial Template Concen
- Page 209 and 210:
Calculating Threshold Cycles A-10 T
- Page 212 and 213:
Importing and Exporting Plate Docum
- Page 214 and 215:
Configuring the Setup Table File wi
- Page 216 and 217:
About the Setup Table File Format S
- Page 218 and 219:
Setup Table Elements (continued) Nu
- Page 220 and 221:
Exporting Plate Document Data Expor
- Page 222 and 223:
Designing TaqMan Assays C In This A
- Page 224 and 225:
Design Probes and Primers The follo
- Page 226 and 227:
Design Tips for Allelic Discriminat
- Page 228 and 229:
Kits, Reagents and Consumables D In
- Page 230 and 231:
Consumables and Disposables The ABI
- Page 232:
TaqMan Pre-Developed Assays and Rea
- Page 236:
Contacting Technical Support F Serv
- Page 239 and 240:
G-2 Limited Warranty Statement No a
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A(continued) Automation Controller
- Page 243 and 244:
I(continued) installing plate adapt
- Page 245 and 246:
Q quantifying probes and primers C-
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Headquarters 850 Lincoln Centre Dri