ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Copying and
Applying Markers to
aPlateDocument
4-10 Run Setup and Basic Operation
IMPORTANT Once copied to the plate document, a marker is no longer linked to the
corresponding entry in the Marker Manager. Therefore, if a marker is modified in the Marker
Manager after it has been copied to a plate document, the marker must be removed from the
plate and copied again to update the plate document with the changes.
To copy and apply markers to the plate document:
Step Action
1 From the Marker Manager dialog box of the SDS software, copy the allelic
discrimination marker to the plate document as follows:
a. Click the marker you want to apply to the plate document.
The software highlights the selected marker.
b. Click Copy to Plate Document.
The software copies the marker and associated detectors to the plate document.
c. Repeat steps a and b to copy additional markers to the allelic discrimination
plate document as needed.
2 Click Done.
The software closes the Marker Manager dialog box.
3 Select the wells containing the assays for a marker you configured in the previous
procedure.
Note For easier selection of plate grid wells, use the Ctrl and Shift keys to select
wells individually or in groups. See page 3-16 for more information.
4 From the marker inspector, click the Use check box of the marker you want to add to
the selected wells.
The software labels the selected wells with the marker and its detectors.
Note The detectors associated with the marker are automatically applied to the
selected wells when the marker is placed in Use.
Detectors associated with
the ‘PDAR CYP 2C9*2’
marker
Use check box for the
‘PDAR CYP 2C9*2’ marker
(selected)
5 If necessary, repeat steps 3 and 4 to assign additional markers to the plate
document.
6 Configure the plate document with detector tasks as explained in
“Step 3 – Configuring the Plate Document with Detector Tasks” on page 4-11.