ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Scrutinizing the
Allele Calls
To analyze allele types:
Step Action
1 Verify the calls for the NTC, Allele X, andAllele Y controls.
a. From the plate grid, select the wells containing the No Template Control samples.
The software highlights the datapoints within the allele plot.
b. Check that the datapoints cluster in the expected position on the plot.
c. If using positive controls, repeat steps a and b for the wells containing the
Allele X, andAllele Y controls.
2 Designate samples that did not cluster tightly as Undetermined.
Samples that did not cluster tightly may:
♦ Contain rare sequence variations
♦ Contain sequence duplications
Allele Y controls
should cluster here
Allele X controls
should cluster here
No Template
Controls should
cluster here
Undetermined
samples
End-Point Analysis 5-13