ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Step 4 – Programming the Plate Document Method
About SDS Methods During a run, the SDS software controls the instrument based on the instructions
encoded within the method of the plate document. Each new plate document
(except allelic discrimination) contains a default method that must be edited for the
specifics of the experiment.
Methods contain the:
♦ Thermal Cycler Conditions
♦ Auto Increment Values
♦ Ramp Rates
♦ Data Collection Options
♦ Reaction Volume Setting
To create a method for… Then…
absolute quantification program the method as explained on page 4-14.
allelic discrimination see page 4-14.
dissociation curve analysis Dissociation curves are preformed as part of a real-time PCR
run (absolute quantification). Therefore, to perform a
dissociation curve analysis do the following:
a. Program the method for the absolute quantification
experiment as explained on page 4-14.
b. Addatemperatureramptothemethodfordissociation
curve analysis as explained on page 4-16.
Run Setup and Basic Operation 4-13