Program - Society of Toxicology

44 th Annual Meeting
and ToxExpo
Program Description
#653 10:45 RISK ASSESSMENT AND SAFETY EVALUATION
OF NANOMATERIALS IN CONSUMER
PRODUCTS. A. B. Santamaria 1 and N. J. Rachman 2 .
1 2 Health Risk, Exponent, Houston, TX and Food and
Chemicals, Exponent, Washington, DC.
Tuesday Morning, March 8
8:30 AM to 11:30 AM
Room RO6
SYMPOSIUM SESSION: NUCLEOCYTOPLASMIC TRAFFICKING
IN MECHANISMS OF TOXICITY
Chairperson(s): Walter Watson, Johns Hopkins Bloomberg School of Public
Health, Baltimore, MD and Richard Pollenz, University of South Florida,
Tampa, FL.
Endorsed by:
Mechanisms SS*
Molecular Biology SS
Student Advisory Committee
#659 10:50 TOXICANT-INDUCED NUCLEAR
TRANSLOCATION OF THIOREDOXIN. W. H.
Watson. Environmental Health Sciences, Johns Hopkins
Bloomberg School of Public Health, Baltimore, MD.
Tuesday Morning, March 8
8:30 AM to 11:30 AM
Room RO3
WORKSHOP SESSION: CURRENT STATUS AND FUTURE
CONSIDERATIONS FOR THE DEVELOPMENT AND VALIDATION
OF IN VITRO ALTERNATIVES TO THE DRAIZE RABBIT EYE TEST
Chairperson(s): William Stokes, National Institute of Environmental Health
Sciences, Research Triangle Park, NC.
Endorsed by:
In Vitro SS*
Regulatory and Safety Evaluation SS
Risk Assessment SS
Student Advisory Committee
TUESDAY
Transport of proteins across the nuclear membrane is tightly regulated by the
nuclear pore, a multisubunit complex consisting of at least 30 proteins. There
are few known examples of toxins or toxicants that act directly at the nuclear
pore to affect transport. However, import or export of specific proteins is a key
component of cellular responses to a variety of toxicants. For example, many
transcription factors are maintained as inactive cytoplasmic pools that can
rapidly translocate to the nucleus upon exposure to toxicants and other stimuli.
Interactions that anchor proteins in either compartment or target proteins to the
nuclear pore may be disrupted (or enhanced) by toxicants or by specific posttranslational
modifications such as phosphorylation or SUMO modification. An
understanding of the factors that control the dynamic localization of proteins
across the nuclear membrane is necessary for the definition of toxicological
mechanisms and physiological pathways.
#654 8:30 NUCLEOCYTOPLASMIC TRAFFICKING IN
MECHANISMS OF TOXICITY. W. H. Watson 1 and
G. H. Perdew 2 . 1 Environmental Health Sciences, Johns
Hopkins Bloomberg School of Public Health,
Baltimore, MD and 2 Center for Molecular Toxicology,
Penn State University, University Park, PA.
#655 8:30 NUCLEOCYTOPLASMIC TRAFFICKING IN
MECHANISMS OF TOXICITY. W. H. Watson.
Environmental Health Sciences, Johns Hopkins
Bloomberg School of Public Health, Baltimore, MD.
#656 8:35 NUCLEAR PROTEIN TRANSPORT: A ROLE IN
REGULATING CELLULAR RESPONSES TO
STIMULI. A. H. Corbett, M. T. Harreman, T. M. Kline
and A. E. Hodel. Biochemistry, Emory University
School of Medicine, Atlanta, GA. Sponsor: W. Watson.
#657 9:20 SPECIES-DEPENDENT DIFFERENCES IN AH
RECEPTOR NUCLEOCYTOPLASMIC
SHUTTLING PROPERTIES. G. Perdew, P.
Ramadoss and B. D. Hollingshead. Center for
Molecular Toxicology and Carcinogenesis and
Department of Veterinary Science, The Pennsylvania
State University, University Park, PA.
#658 10:05 SUMO MODIFICATION AND REGULATION OF
NUCLEAR TRANSPORT. M. J. Matunis and H.
Zhang. Biochemistry and Molecular Biology, Johns
Hopkins University, Baltimore, MD. Sponsor: W.
Watson.
The Draize rabbit eye test has been used as the standard test method for
assessing ocular irritation and corrosivity potential of chemicals and products
for over 60 years. Largely due to the pain and discomfort associated with this
test, there have been extensive efforts to develop and validate alternative test
methods that would partially or fully replace the current regulatory version of
the Draize rabbit eye test. These efforts are also being driven by the 7th
Amendment to the European Union Cosmetics Directive that will ban the use of
animals for ocular testing by 2009 and pressure to minimize or avoid animal use
for the European Union Chemicals Policy Initiative (REACH). This workshop
will address scientific and regulatory considerations for developing and validating
in vitro test methods that will refine, reduce, and replace the Draize rabbit
eye test. ICCVAM, NICEATM, and ECVAM have recently initiated collaborations
to assess the usefulness of currently available in vitro ocular toxicity
methods and to review the state-of-the-science with regard to in vitro test
methods. These efforts include an evaluation of several in vitro test methods for
their ability to detect severe ocular irritants, an assessment of the performance
characteristics of the in vivo rabbit eye test, and a review of the current status of
methods for identifying nonirritants and mild to moderate irritants. Current and
future challenges in validating alternative methods and approaches to evaluate
ocular toxicity will be presented including the need for high quality reference
data to assess test method performance.
#660 8:30 CURRENT STATUS AND FUTURE
CONSIDERATIONS FOR THE DEVELOPMENT
OF IN VITRO ALTERNATIVES TO THE DRAIZE
RABBIT EYE TEST FOR ASSESSING OCULAR
IRRITANTS. T. Hartung 1 and W. S. Stokes 2 . 1 ECVAM,
Ispra, Italy and 2 NICEATM, NIEHS/NIH/DHHS,
Research Triangle Park, NC.
#661 8:40 CURRENT STATUS AND FUTURE
CONSIDERATIONS FOR ALTERNATIVE
METHODS TO REFINE, REDUCE, AND
REPLACE ANIMAL USE FOR OCULAR SAFETY
TESTING. W. S. Stokes. NICEATM,
NIEHS/NIH/DHHS, Research Triangle Park, NC.
#662 9:10 THE PERFORMANCE CHARACTERISTICS OF
THE IN VIVO RABBIT EYE TEST. J. H. Haseman 1 ,
N. Y. Choksi 2,3 , C. Inhof 2,3 , J. Truax 2,3 , R. R. Tice 2,3 and
W. S. Stokes 3 . 1 Consultant, NIEHS/NIH/DHHS,
Research Triangle Park, NC, 2 ILS, Inc., Research
Triangle Park, NC and 3 NICEATM,
NIEHS/NIH/DHHS, Research Triangle Park, NC.
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SOT’s 44 th Annual Meeting