23. Ulusal Biyokimya Kongresi Ãzel SayÄ±sÄ± - TÃ¼rk Biyokimya Dergisi

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XXIII. ULUSAL B‹YOK‹MYA KONGRES‹ 29 Kasım - 2 Aralık 2011 Hilton Hotel - Adana 23. Ulusal Biyokimya Kongresi, Adana [23 rd National Biochemistry Congress, Adana / TURKEY] İÇİNDEKİLER P. 031 / KOMPLİKASYONLU VE KOMPLİKASYONSUZ TİP 2 DİYABETLİ HASTALARDA APOLİPOPROTEİN B-100 R3500Q MUTASYONUNUN ARAŞTIRILMASI Ebubekir BAKAN, Adem GÜVENÇ, Abdulkadir YILDIRIM, Akar KARAKOÇ, Vefa YANMAZ Atatürk Üniversitesi, Biyokimya, Erzurum Apolipoprotein B-100 (B-100), düşük yoğunluklu lipoprotein (LDL)’nin bir bileşimidir ve LDL reseptörü için ligant olarak görev yapar. Bundan dolayı Apo B genindeki mutasyonlar, fonksiyonel faaliyetlerini önemli ölçüde değiştirerek orta ya da ciddi düzeyde hiperkolesterolemiye ve sonuçta erken ateroskleroza neden olur. Bu çalışmada Tip 2 diyabetli hastalarda Apo B-100 R3500Q mutasyonunun araştırılması amaçlandı. Yirmi altısı komplikasyonlu olmak üzere toplam 46 Tip 2 diyabetli hasta çalışmaya dahil edildi. Periferik kandan elde edilen DNA örneklerinden PCR-strip hibridizasyon tekniği ile Apo B R3500Q mutasyon analizi ve konvansiyonel yöntemlerle serum glukoz ve lipit profili analizleri yapıldı. Herhangi bir komplikasyonu (makro- ya da mikro-vasküler) olan ve olmayan Tip 2 diyabetli hasta grupları karşılaştırıldığında, Apo B-100 R3500Q mutasyonu görülme sıklığı açısından gruplar arasında istatistiksel olarak anlamlı bir fark bulunmamıştır. P. 031 / THE INVESTIGATION OF APO B-100 R3500Q MUTATION IN TYPE 2 DIABETICS WITH AND WITHOUT COMPLICATION Ebubekir BAKAN, Adem GÜVENÇ, Abdulkadir YILDIRIM, Akar KARAKOÇ, Vefa YANMAZ Medical Biochemistry, Faculty of Medicine, Ataturk University, Erzurum Apo B-100 is an interior combination of LDL and functions as a ligand for LDL receptor. Therefore, the mutations in the Apo B gene modify significantly their functional activities and delay the circulation of LDL particles by reducing the binding the LDL receptor, resulting in, generally, serious or mild hypercholesterolemia and in early risk of atherosclerosis. In this study, the investigation of the mutation, Apo B-100 R3500Q, in patients with Type 2 diabetes was aimed. Total 46 patients with type 2 diabetes were included in the study (26 having complications). After DNA samples were extracted from peripheral blood, the Apo B R3500Q mutation analysis was performed by using the PCR-strips Hybridization technique. Serum glucose levels and lipid profile were analyzed with conventional methods. When the type 2 diabetic group having any complications (macro or micro vascular) is compared with that no complications, no significant diversity in the possibility of R3500Q detection was found. CONTENTS Turk J Biochem, 2011; 36 (S2) http://www.TurkJBiochem.com
XXIII. ULUSAL B‹YOK‹MYA KONGRES‹ 29 Kasım - 2 Aralık 2011 Hilton Hotel - Adana 23. Ulusal Biyokimya Kongresi, Adana [23 rd National Biochemistry Congress, Adana / TURKEY] İÇİNDEKİLER P. 032 / TİP 2 DİYABETTE PLAZMA VE ERİTROSİTLERDE KROM, ÇİNKO, BAKIR VE MAGNEZYUM SEVİYELERİNİN AÇLIK KAN ŞEKERİ, İNSÜLİN VE HBA1C İLE KARŞILAŞTIRILMASI Elif POLAT 1 , Yaşar Nuri ŞAHİN 1 , Asuman ÖZKAN 2 1 Atatürk Üniversitesi, Tıbbi Biyokimya, Erzurum 2 Bölge Eğitim ve Araştırma Hastanesi, Biyokimya, Erzurum Diyabetes mellitus çağımızın en yaygın hastalıklarından biridir. Çok çalışılan ve halen bilinmeyenleri hakkında çalışmalar yapılmakta olan bir hastalıktır. Diyabet insan organizmasında bulunan miktarları az olmakla beraber, fizyolojik önemleri yüksek olan eser elementleri de etkilemektedir. Bu çalışmada tip 2 diyabette HbA1c, açlık kan şekeri ve insülinin; çinko, bakır, magnezyum ve krom gibi eser elementlerle ilişkisinin ve korelasyonunun incelenmesi amaçlanmıştır. Bu çalışma, 20 tip 2 diyabet tanısı almış ve 20 sağlıklı kontrol grubu olmak üzere toplam 40 kişi üzerinde yürütülmüştür. Elde edilen analiz sonuçlarının istatistiği için SPSS for Windows 19.0 kullanıldı Bu çalışmada tip 2 diyabetiklerde HbA1c (türbidimetrik inhibisyon immunoassay yöntemi ile), glukoz (hekzokinaz yöntemi ile), insülin (kelimilüminesans immunometrik yöntem ile), plazma ve eritrositte Zn, Cu, Mg (alevli atomik absorbsiyon), Cr (elektrotermel atomik absorbsiyon spektrofotometrik yöntemle) ölçüldü. Tip 2 diyabette HbA1c, glukoz ve insülin seviyeleri kontrol grubunda anlamlı olarak düşüktü. Kontrol grubu ve hasta grubunda plazma Zn ve plazma Cu ile eritrosit Cu seviyeleri arasında anlamlı farklılık yoktu. Kontrol grubunda plazma Mg, plazma Cr, eritrosit Zn eritrosit Mg ve eritrosit Cr seviyeleri hasta grubundaki bu değerlerden anlamlı olarak daha düşüktü. HbA1c ile glukoz ve insülin arasında pozitif korelasyon vardı. HbA1c ile plazma ve eritrosit Cr arasında ise negatif korelasyon bulundu. P. 032 / COMPARISON OF THE LEVELS OF CHROMIUM, ZINC, COPPER AND MAGNESIUM IN PLASMA AND ERYTHROCYTES WITH FASTING BLOOD GLUCOSE, INSULIN AND HBA1C IN TYPE2 DIABETES MELLITUS Elif POLAT 1 , Yaşar Nuri ŞAHİN 1 , Asuman ÖZKAN 2 Biochemistry, Ataturk University, Erzurum Biochemistry, Region Education and Research Hospital, Erzurum Diabetes mellitus is one of the most common diseases of our time. Although there have been lots of studies on the pathogenic processes involved in the development of diabetes, there are still many mysteries concerning this disorder. It is aimed to investigate correlation and relationship of HbA1c, fasting blood glucose, and insulin levels with zinc, copper, magnesium and chrome levels in type2 diabetes. This study has been carried on 40 subjects in which 20 with type2 diabetes and 20 healthy control group. The results of analyses were calculated with the help of SPSS (Vers 19.0) software package. In this study, we analyzed the the levels of the HbA1c, (with Turbidimetric inhibition immunoassay) glucose, (with Hexokinase method) insulin (with Chemiluminescense Immunmetric Assay) in the patients suffer from type 2 diabetes. Additionally blood plasma and erithrocytes were screened to determine the levels of zinc (Zn), copper (Cu), magnesium (Mg) and chrome (Cr) elements by using Atomic Absorption Spectrophotometer. Specifically Chrome (Cr) was the only element analyzed by Electrothermal Method while the remaining elements were measured with the help of Flamed Atomization Method. HbA1c, insuline, glucose levels in type 2 diabetes were significantly increased in control group. There were no significant different in among plasma Zn and Cu and erythrocyte Cu levels in patient and contol groups. Plasma Mg, plasma Cr, erythrocyte Zn, erythrocyte Mg and erythrocyte Cr levels in control group was significantly higher than those of patient group.There was a significant positive correlation between glucose and insuline levels with HbA1c levels. The negative correlation was found between plasma and erythrocyte Cr with HbA1c. CONTENTS Turk J Biochem, 2011; 36 (S2) http://www.TurkJBiochem.com
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23. Ulusal Biyokimya Kongresi Ãzel SayÄ±sÄ± - TÃ¼rk Biyokimya Dergisi

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23. Ulusal Biyokimya Kongresi Ãzel SayÄ±sÄ± - TÃ¼rk Biyokimya Dergisi