23. Ulusal Biyokimya Kongresi Özel Sayısı - Türk Biyokimya Dergisi

XXIII. ULUSAL B‹YOK‹MYA KONGRES‹
29 Kasım - 2 Aralık 2011
Hilton Hotel - Adana
23. Ulusal Biyokimya Kongresi, Adana [23 rd National Biochemistry Congress, Adana / TURKEY]
İÇİNDEKİLER
PROTEOMIC EVALUATION OF THORACIC AORTIC ANEURYSM
Ahmet TARIK BAYKAL
Proteomics Core Laboratory, Technological and Scientific Research Council
of Turkey Marmara Research Center Genetic Engineering and Biotechnology
Institute Gebze- Kocaeli, Turkey
CONTENTS
DAVETLİ KONUŞMACI ÖZETLERİ
Aortic aneurysms (AA), a common disease affecting up to ~9% of individuals above
the age 65, can be characterized as localized degeneration around the aorta leading to
weakening and widening of the vessel. As predicted, the most dangerous complication
associated with formation of an aneurysm is its rupture as a result of advanced
weakening. While the exact mechanisms are yet to be determined, the current studies
indicate that the degradation of extracellular matrix proteins and apoptosis of vascular
smooth muscle cells (VSMC) within the aorta may result in extendibility, dilatation
and rupture of the vessel. As the older population increases in Europe and Turkey,
early diagnosis and treatment is very important in diseases. Within the aortic wall
can be found different types of cells such as endothelial cells, fibroblasts, infiltrating
immune system cells and smooth muscle cells (SMC). Among these, SMCs appear as
an important factor in disease development as numerous molecular changes have been
reported to occur in these cells. Most of these studies involve either investigation of
expression changes at single target level in SMCs or global analyses in the whole aortic
tissue. In order to determine which proteins are important in aneurysm development,
we focused on SMCs and performed comparative proteomic analyses using isolated,
cultured SMCs from normal versus thoracic aneurismal aortic wall. We hypothesized
that cultured SMCs still represent tissue characteristics and investigation of molecular
changes in this sub-fraction of aorta should be able to better reveal the underlying
reasons for this disease. Label-free LC-MS/MS analysis of cell extract resulted in the
identification of about 800 proteins. The application of replicate filter yielded 140
proteins which are detected in every sample. 20 proteins were found to be statistically
important. Among the identified proteins calmodulin, caldesmon, and peroxiredoxin
play important roles in inflammation, SMC contraction, apoptosis and cellular toxicity.
We will attempt to build a multi protein model to shed light on the cellular mechanism
of aneurysm.
ABSTRACTS OF INVITED LECTURES
Turk J Biochem, 2011; 36 (S2)
http://www.TurkJBiochem.com