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CONTRIBUCION A LA BIOLOGIA E INMUNOLOGIA DE Toxocara ...

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Glucosa • í g<br />

CO HNa 0,35 g<br />

3<br />

destilada c.s.p 1.000 ml<br />

Disolver por separado Cl 2Ca y SO4Mg.7H20 para evitar<br />

precipitaciones; esterilizar por filtración y conservar a 49C.<br />

* MEZC<strong>LA</strong> SULFOCROMICA<br />

bicromato potásico<br />

SO 1-1 concentrado<br />

42<br />

1120 destilada c.s.p.<br />

* SEPARADOR <strong>DE</strong> LINFOCITOS (Linfomatre)<br />

10 g<br />

200 ml<br />

1.000 ml<br />

* MEDIO MíNIMO ESENCIAL <strong>DE</strong> EAGLE CON SALES <strong>DE</strong> EARLE (Cultek<br />

‘~ GLUTAMINA<br />

* n-HEXANO<br />

*<br />

*<br />

*<br />

ALBUMINA SERICA BOVINA -B.S.A.- (Sigma)<br />

REACTIVO<br />

ADYUVANTE<br />

<strong>DE</strong> BRA<strong>DE</strong>ORD (Biorad)<br />

COMPLETO <strong>DE</strong> FREUND (Biomérieux)<br />

* AiDYUVANTE INCOMPLETO <strong>DE</strong> FREUND (Biomérieux<br />

169

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