Pathologica 4-07.pdf - Pacini Editore

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ceptors (TβRI, TβRII) and involucrin with the clinico-pathological
characteristics of the oral squamous cell carcinomas.
Moreover, we investigated on two squamous carcinoma cell
lines the responsiveness to TGF-b1 treatment in relation to
the baseline expression patterns of TbRI and TbRII receptors.
Methods. Immunohistochemistry was performed from 22
oral carcinomas and their corresponding normal mucosae using
antibodies against TGF-β1, TβRI, TβRII and involucrin.
TGF-β1, TβRI and TβRII expression levels were also evaluated
by Western blot analysis using specific antibodies.
Results. TGF-β1, TGF-β receptors and involucrin were differentially
expressed in neoplastic tissues as compared to the
surrounding apparently unaffected normal epithelia. The
TGF-β1 system and involucrin were expressed in normal epithelia
of all patients. In contrast, in the neoplastic tissues a
loss of expression of TGF-β1, TGF-β1 receptors and involucrin
was observed. The reduction of the expression was correlated
with the clinical stage of disease, decreasing progressively
from stage I to stage IV. In addition, a correlation between
TGF-β1 system molecules/involucrin expressions and
grade of differentiation of the tumor was observed. In all cases,
TGF-β1, TGF-β1 receptors and involucrin expressions
were significantly diminished in G3 and G2 tumors as compared
to G1 lesions. Moreover, our results demonstrated a reduced
and a lack of TbRI expression in the oral squamous
carcinoma cell lines Cal27 and FaDu respectively. In addition,
a significant decrease of TbRII expression, as compared
to Cal27 cells, was shown in FaDu cell line. The decreased
expression of TbRII and the absence of TbRI, could account
for the resistance of FaDu cells to the growth-inhibiting effect
of TGF-b1 in vitro treatments.
Conclusion. In OSCC, the loss of TGF-β1, TGF-β1 receptors
and involucrin expression significantly correlated with
the grade of differentiation and with the clinical stage of the
tumor. Thus, the decrease of expression of the TGF-β1 system
molecules, associated to advanced and more aggressive
tumors, suggests a functional role of these molecules in the
oral tumor progression. Moreover, results from in vitro studies
suggest that alterations in TGF-b1 receptors expression
could represent one of the mechanisms that allow cells to
evade TGF-b1-induced growth arrest.
P16 expression in odontogenic tumors
L. Artese, A. Piattelli, C. Rubini * , V. Perrotti, G. Iezzi, M.
Piccirilli, F. Carinci **
Department of Stomatology and Oral Science, University
“G. d’Annunzio” of Chieti-Pescara, Chieti, Italy; * Department
of Pathology, University of Ancona, Italy; ** Departemnt
of D.M.C.C.C., Section of Maxillofacial Surgery, University
of Ferrara, Italy
Introduction. The odontogenic tumors (OTs) are uncommon
lesions. The p16 gene was discovered as a multiple tumor
suppressor gene, which directly regulates the cell cycle and
inhibits cell division. The aim of the present study was to examine
the expression of p16 in OTs with a low and a high risk
of recurrences, to clarify the possible role of this factor in the
invasiveness of these tumors.
Methods. The tissues of 36 OTs were evaluated: 2 calcifyng
cystic OTs, 2 odontogenic fibromas, 9 ameloblastomas-unicystic
type, 4 ameloblastomas-extraosseous/peripheral type,
19 ameloblastomas-solid/multicystic type To evaluate the
p16 expression a mean percentage of positive cells was de-
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termined, derived from the analysis of 100 cells in ten random
areas at x 40 magnification. To better evaluate the relationship
between p16 expression and prognosis, the tumors
were divided in 2 groups according to the clinical behavior.
A. OTs with low risk of recurrences (i.e. calcifyng cystic
OTs, odontogenic fibromas, ameloblastomas-unicystic type,
ameloblastomas-extraosseous/peripheral type); B. OTs with
high risk of recurrences (i.e. ameloblastomas-solid/multicystic
type).
Results. P16 was expressed in all the OTs but the location of
the expression was different. Group A: the positivity was expressed
at the level of the stellate reticulum cells in 15 cases
(88.23%), while these cells were negative in 2 case (11.76%).
Columnar/cuboidal peripheral cells were almost negative in
all cases. Group B: it was possible to observe a prevalent positivity
of the stellate reticulum cells in 12 cases (85.71%),
while in 2 cases a prevalent negativity (14.28%) was present.
Columnar/cuboidal peripheral cells were positive in 6 cases
(42.85%), while were prevalently negative in 8 cases
(57.14%). Statistically difference was found in p16 expression
of peripheral cells with an increase of the expression in
group A compared to group B (p < 0.05). Statistically significant
difference was found in p16 positive expression of the
central cells of OTs with a decrease of the expression in
group A compared to group B (p < 0.05).
Conclusion. The study show a correlation between the p16
expression and biological behavior of OTs. The peripheral
portion of the tumors (i.e. the areas of tumor growth) shows
a statistically significant higher quantity of p16+ cells in the
group of tumors with a high risk of recurrences. p16 can be
considered an useful marker to predict the recurrence and aggressive
behavior of OTs.
Sialolipoma della sottomandibolare: case
report
P. Parente, F. Castri, I. Pennacchia, G. Bigotti, F. Federico,
A. Coli, G. Massi
Istituto di Anatomia Patologica, Università Cattolica del Sacro
Cuore, Roma
Introduzione. Le neoplasie a componente adipocitaria delle
ghiandole salivari sono rare (0,5% circa) e sono rappresentate
dal lipoma e dalle forme miste. Nel 2001 Nagao descrive
una nuova variante istologica denominata sialolipoma. Riportiamo
il primo caso di sialolipoma a insorgenza nella
ghiandola sottomandibolare.
Case report. Una donna di 77 anni viene operata per l’asportazione
di una neoformazione solida non dolente in zona
retromandibolare adiacente alla ghiandola salivare, ben capsulata
e non infiltrante le strutture anatomiche circostanti.
Materiali e metodi. Il materiale è stato tutto incluso e sezionato
e colorato in Ematossilina Eosina. All’esame macroscopico
la neoplasia era compatta, capsulata e omogenea e giallastra
al taglio, del diametro di 2 cm, adiacente ma non infiltrante
la ghiandola salivare. Istologicamente la neoformazione
era composta da una proliferazione di adipociti maturi tipici,
privi di mitosi e necrosi, tra i quali erano rare strutture
ghiandolari con aspetti di differenziazione oncocitaria, circondata
da una capsula fibrosa dalla quale partivano sottili
introflessioni conferenti alla neoplasia un aspetto settato.
Discussione. Le neoplasie con componente adipocitaria delle
ghiandole salivari sono il lipoma e i tumori misti. Nagao
descrive un istotipo particolare caratterizzato dalla presenza