Pathologica 4-07.pdf - Pacini Editore

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un coinvolgimento, in particolare di BACE2, sia nei disturbi
della migrazione sia nella patogenesi delle alterazioni neuropatologiche
dell’AD.
Pathology of IUGR by study of growth
factors and apoptosis markers
M. Mangiapia, G. Maruotti * , G. Merone, I. Ventre, F. Ventre,
F.P. D’Armiento, M. D’Armiento
Dipartimento di Scienze Biomorfologiche e Funzionali, Sezione
di Anatomia Patologica, Università “Federico II” di
Napoli; * Dipartimento di Ginecologia ed Ostetricia, Università
“Federico II” di Napoli, Italia
Introduction. Intrauterine growth restriction (IUGR) could
be associated with congenital anomalies, frequently with vascular
placental pathology, sometimes with maternal pathology
(early-onset preeclampsia).
We will study organs and placentas by perinatal autopsies
performed by a standardized autoptic protocol (by malformated
and not cases) of IUGR cases and control cases.
For each case, we will confirm IUGR and diagnosis paraffin
blocks of representative areas of the lesion will be selected
(principally brain, kidney, lung – markers of development and
maturation – and placenta) to evaluate morphogenetic alterations
and apoptosis of placenta of fetus and of pregnancies
with preeclampsia/liveborn IUGR and controls.
Methods. Organs and pacental sections were obtained from
women with early-onset preeclampsia/liveborn IUGR (n. 11)
and in controls (n. 18). Trophoblast’s apoptosis into placenta
was quantified evaluating bcl-2, PARP 1 and Apaf-1 apoptosis
markers; placental’s morphogenetic alterations was evaluated
with HGF/c-met/STAT3 cascade (by immunohistochemistry
and RNA extraction).
Results. We found two patterns of placental development IU-
GR-related: 1) accelerated maturation and 2) delayed maturation
(“terminal villous deficiency” mixed with mild persisting
immaturity). Unexpectly, trophoblast apoptosis was
not increased in preeclampsia/IUGR significantly (p > 0.05);
HGF/c-met/STAT3 cascade was completely altered in severe
IUGR patients with placentas showing terminal villous deficiency
(p < 0.05).
Conclusions. This review focuses on different roles of trophoblast
apoptosis into placenta and show the role of HGF/cmet/STAT3
cascade in the onset of pregnancies with
preeclampsia/IUGR.
Expression of homeobox genes in placentas
with thrombophilias: significant
overexpression of HOX-A2 gene
F. Castiglione, A.M. Buccoliero, F. Garbin, C.F. Gheri, F.
Mecacci * , D. Moncini, D. Rossi Degl’Innocenti, M. Paidas
** , G.L. Taddei
Dipartimento di Patologia Umana ed Oncologia Azienda
Ospedaliera Universitaria Careggi, Firenze; * Dipartimento
di Ginecologia e Perinatologia e Fisiopatologia della Riproduzione
Azienda Ospedaliera Universitaria Careggi, Firenze;
** Yale University USA
Homeobox genes (HOX) are a family of regulatory genes
that encode nuclear proteins (homeoproteins) which act as
POSTERS
transcription factors. These homeoproteins have the autoregulation
ability and also regulate other homeobox genes.
Homeoproteins can either activate or inactivate downstream
genes acting on transcription phase.
Although initially described as control genes regulating pattern
formation in developing embryos, homeobox genes are
now known to be expressed in specific adult tissues: placenta
and cancer. In addition they play a critical role in the regulation
of cellular differentiation at many levels 1 2 .
The aim of this study was to investigate the role of HOX
genes A2, A4, A9, C6 in human placenta.
HOX genes mRNA expression was assessed in fresh tissue of
human placenta from 12 consecutive placentas of thrombophilic
women and from 10 sample of normal human placenta.
All patients have a term birth. All the women were
treated at the Department of Gynecology, Perinatology and
Reproductive Medicine of the University of Florence –
School of Medicine, Florence, Italy, from August 2005 to
January 2006. The patients age was from 28 to 37 years (median,
32.5 years; mean, 32.1 years). RNA amplification was
assessed by reverse-transcription polymerase chain reaction
assay and measured quantitatively. mRNA was successfully
extracted in all cases.
Expression of HOX-A2, HOX-A4, HOX-A9 and HOX-C6
genes was detected respectively in 66%, 50%, 91%, 100% of
the placentas with thrombolphilias, and respectively in 50%,
20%, 90%, and 90% of physiological placentas. HOX-A2 expression
showed a significant correlation between placentas
with thrombophilias and the mean of physiological placentas
(p = 0,05 Kruskal-Wallis test), whereas no correlation was
found in HOX-A4, HOX-A9 and HOX-C6 genes expression
between placentas with thrombophilias and the mean of
physiological placentas.
Until now, only this report investigated the quantitative expression
of HOX genes in placentas using PCR Real-time.
The presence of HOX genes and the correlation of HOX-A2
gene expression levels between placentas with thrombophilias
and physiological placentas support the hypothesis that
this genes may be involved in the regulation of cellular differentiation,
playing a critical role.
These results suggest to deepen these researches with further
investigation, in order to estimate a possible prognostic value
of HOX genes expression in thrombophilic placenta.
References
1 Zhang X, Zhu T, Chen Y, et al. Human growth hormone-regulated
HOXA1 is a human mammary epithelial oncogene. J Biol Chem
2003;278:7580-90.
2 Amesse LS, Moulton R, Zhang YM, et al. Expression of HOX gene
products in normal and abnormal trophoblastic tissue. Gynecol Oncol
2003;90:512-8.
Linfangioma neonatale: esordio con
insufficienza respiratoria. Caso clinico
F. Tallarigo, I. Putrino, S. Squillaci ** , U.P. Corapi * , V. Poerio
* , M. Bisceglia *
U.O. Anatomia Patologica e Citodiagnostica, * U.O. Patologia
Neonatale TIN “Capotorti”, Ospedale “S. Giovanni di
Dio”, Crotone; ** U.O. Anatomia Patologica e Citodiagnostica
Ospedale di Vallecamonica, Esine (BS)
Introduzione. I Linfangiomi sono anomalie del sistema linfatico,
presenti già alla nascita, associate ad alterazioni della di-