Effet chez le porcelet d'une exposition à un régime co-contaminé en ...

These are not the final page numbersMol. Nutr. Food Res. 2011, 55, 1–11 7LIVER76cABLesional score54321abb,c0LUNGKIDNEYCDLesional scoreLesional scoreE F a9b,c c87654 a,b3a2103 bb2b10Control DON FB DON+FBAnimal treatmentsFigure 1. Individual and combinedeffects of DON and FB onliver, lungs and kidneys. Pigsreceived a control diet (&), or aDON-contaminated diet ( ), oran FB-contaminated diet ( )or a diet contaminated withboth toxins (&). (A) Hepatocytecytoplasmatic vacuolizationand (B) hepatocytemegalocytosis (arrow). HE40 . (C) BALT depletion andperibronchiolar hemorrhage.HE 10 and (D) Alveolaredema. HE 40 . (E) Cytoplasmaticvacuolization of tubularcells and mitosis (arrow) and(F) nuclear change (arrow)in tubular cells. HE 40 .Lesion scores were establishedafter histological examinationaccording to the severity andthe extent of the lesions. Valuesare mean7SEM for fiveanimals.4 DiscussionIn the present 5-wk study, piglets were exposed to low dosesof two major Fusarium mycotoxins, DON and FB, at levelscommonly found in crops. Most of the current dataconcerning the effects of DON or FB on animals, includingrodents, have been obtained using highly mono-contaminatedfeeds [9, 12, 13, 28]. It was thus of interest to determinethe effect of ingestion of feeds contaminated with lowlevel of these toxins, present alone or in combination, onzootechnical, hematological, biochemical, histopathologicaland immune parameters of piglets.We did not observe any effect of mycotoxin-contaminateddiets (DON, FB, DON1FB) on the body weight gain ofthe animals. Considering the low contamination levelswe used, these results are not surprising. Indeed, noeffect on body weight gain has been reported in pigsand in poultry fed with up to 70 mg FB/kg feed [18, 29]. Theeffects of DON on body weight gain are more controversial,especially in pigs. Some studies indicate that dietaryconcentrations of DON above 1–2 mg/kg have an effect onweight gain, whereas in other studies no effect is observedfor up to 4.5 mg DON/kg feed [30]. A weight gain reductionhas also been described when DON and FB were giventogether to growing barrows [18]. However, in this study, thedose of FB was ten-fold higher than the one used in thepresent experiment.Exposure of piglets to low doses of either DON or FB didnot have a major impact on the hematological andbiochemical parameters investigated. For blood hematology,only a reduction in neutrophil numbers was noticed in FBexposedpiglets. This observation is in relation with the& 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheimwww.mnf-journal.com