Effet chez le porcelet d'une exposition à un régime co-contaminé en ...

TRAVAIL EXPERIMENTALbecause of differences in the metabolism of DON. Chronic low dietary concentrations induceanorexia, decreased weight gain, and immune alterations, while acute higher doses induce vomiting,hemorrhagic diarrhea and circulatory shock (Rotter et al., 1996a). At the cellular level, the maineffect is inhibition of protein synthesis via binding to the ribosomes. Low exposure to DON wasshown to upregulate expression of cytokines and inflammatory genes with concurrent immunestimulation, whereas high exposure promoted leukocyte apoptosis associated with immunesuppression (Pestka et al., 2004).The purpose of this study was to compare the effects of low doses of DON and FB in pigs whenfed individually and in combination with particular emphasis on their effects on the intestine. Theexperimental design was a factorial assay including control feed and feed contaminated with 3 and 6mg/kg DON and FB individually and in combination, respectively. These contamination levelscorrespond to levels that frequently occur naturally in cereals. We investigated the effect of DON andFB1 on intestine morphology, on the expression of tight junction as well as on the intestinalexpression of cytokines.111