Effet chez le porcelet d'une exposition à un régime co-contaminé en ...

TRAVAIL EXPERIMENTALABSTRACTFumonisins, produced by Fusarium verticillioides, are of major worldwide concern in terms ofubiquitous and frequency distribution, and toxicity. The need to restrict in feeds the levels offumonisins, and thereby to ensure animal health and productivity, is a continuous challenge.Biotechnological approaches through microorganisms or enzymes can provide an alternativesolution. In this study, the comparative toxicity of fumonisin B1 (FB1), the predominant fumonisins inthis family, and its hydrolyzed form (HFB1) was investigated in swine over a short-term trial. Eighteen5-week-old animals were orally and daily treated for two weeks with tree different solutions, acontrol buffer solution, a FB1 solution (2 mg/kg body weight/day) and a HFB1 solution (equimolar to2 mg FB1/kg body weight/day). The HFB1 solution was initially obtained after a deesterificationreaction on FB1 solution, through the use of a carboxylesterase. Results were reported in terms ofliver and intestinal toxicity. Besides, exposure was assessed by determining the sphingoid basescontent in plasma and liver samples. On contrary to FB1, HFB1 did not trigger hepatotoxicty asrevealed by biochemical compounds, inflammatory markers and microscopical lesions. Similarly,HFB1 did not impair the intestinal integrity and intestinal immune system, as evaluated in differentsegments of gastro-intestinal tract by villi and lesions characterization and cytokines expression.These results are supported by the ratio of sphinganine and sphingosine in biological samples, whichwere not affected at intermediate and end points of the in vivo experiment. It can be concluded thathydrolysis of FB1 strongly reduced toxicity in piglets.127