Effet chez le porcelet d'une exposition à un régime co-contaminé en ...

Table 10 : Origins and dilutions of primary antibodies used for detecting tight junction proteins andβ-actin by Western blottingProtein Origin DilutionE-Cadherin (24E10) Rabbit mAbRabbit anti-Occludin (672381A)β-actin mAB MOUSE (8H10D10)Cell Signaling Technology® - Massachusetts, USA(ref. 3195)Invitrogen Corporation® - California, USA(ref. 71-1500)Cell Signaling Technology® - Massachusetts, USA(ref. 3700)1:5001:5001:1000Table 11 : Nucleotide sequences of primers for real-time PCRGENE PRIMER SEQUENCE GENBANK NO. REFERENCESRPL32F (300 nM) TGCTCTCAGACCCCTTGTGAAGR (300 nM) TTTCCGCCAGTTCCGCTTANM_001001636 Pinton et al.(2010)β2-μglobulin F (900 nM) TTCTACCTTCTGGTCCACACTGAR (300 nM) TCATCCAACCCAGATGCANM_213978Devriendt etal. (2009)IL-12p40F (300 nM) GGTTTCAGACCCGACGAACTCTR (900 nM) CATATGGCCACAATGGGAGATGNM_214013Devriendt etal. (2009)IL-8F (300 nM) GCTCTCTGTGAGGCTGCAGTTCR (900 nM) AAGGTGTGGAATGCGTATTTATGCNM_213867Grenier et al.(2011)IL-1βF (300 nM) GAGCTGAAGGCTCTCCACCTCR (300 nM) ATCGCTGTCATCTCCTTGCACNM_001005149 Devriendt etal. (2009)MIP-1βF (300 nM) AGCGCTCTCAGCACCAATGR (300 nM) AGCTTCCGCACGGTGTATGAJ311717Grenier et al.(2011)IL-6IFN-γTNF-αIL-2IL-10F (300 nM) GGCAAAAGGGAAAGAATCCAGR (300 nM) CGTTCTGTGACTGCAGCTTATCCF (300 nM) TGGTAGCTCTGGGAAACTGAATGR (300 nM) GGCTTTGCGCTGGATCTGF (300 nM) ACTGCACTTCGAGGTTATCGGR (300 nM) GGCGACGGGCTTATCTGAF (300 nM) GCCATTGCTGCTGGATTTACR (300 nM) CCCTCCAGAGCTTTGAGTTCF (300 nM) GGCCCAGTGAAGAGTTTCTTTCR (300 nM) CAACAAGTCGCCCATCTGGTNM_214399NM_213948NM_214022AY294018NM_214041Grenier et al.(2011)Royaee et al.(2004)Meissonnier etal. (2008)Meurens et al.(2008)Present study