Effet chez le porcelet d'une exposition à un régime co-contaminé en ...
Effet chez le porcelet d'une exposition à un régime co-contaminé en ...
Effet chez le porcelet d'une exposition à un régime co-contaminé en ...
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TRAVAIL EXPERIMENTALRESULTS1) Histomorphometrical analysisSamp<strong>le</strong>s of jej<strong>un</strong>um and i<strong>le</strong>um were <strong>co</strong>l<strong>le</strong>cted for histomorphometrical analysis. Pig<strong>le</strong>ts fed diets<strong>co</strong>ntaminated with my<strong>co</strong>toxins showed mild to moderate intestinal <strong>le</strong>sions. The main histologicalchanges observed were lymphatic vessel dilation, mild eosinophil granulation within the cytoplasm of<strong>en</strong>terocytes, and promin<strong>en</strong>t lymphoid follic<strong>le</strong>s. The <strong>le</strong>sional s<strong>co</strong>re increased for animals fed<strong>co</strong>ntaminated diets (Figure 5).Changes in villous height and crypt depth are indicative of <strong>en</strong>terocyte loss and impairedabsorption of nutri<strong>en</strong>ts. As shown in Figure 5, villi height decreased significantly in the jej<strong>un</strong>um of theanimals that received DON and DON+FB wh<strong>en</strong> <strong>co</strong>mpared with <strong>co</strong>ntrols. Focal apical necrosis of villi(Figures 5C and 5D) was also observed in the intestine of pig<strong>le</strong>ts fed my<strong>co</strong>toxins diets. No change incrypt depth was observed in any intestinal region. Gob<strong>le</strong>t cells synthesize and secrete mucin, which isinvolved in gut physiology. The number of gob<strong>le</strong>t cells decreased (Figures 5F and 6) significantly inthe DON- and FB+DON-treated animals in the jej<strong>un</strong>um and i<strong>le</strong>um.Inflammatory infiltrate of lymphocytes, plasma cells and eosinophils was observed in all regions ofthe intestine. In the groups treated with my<strong>co</strong>toxins, a reduction in lymphocytic infiltrate wasobserved in all regions of the intestine. However, only the jej<strong>un</strong>um was significantly affected (Figure6). The number of eosinophils in the lamina propria decreased significantly in the i<strong>le</strong>um of the groupthat received DON+FB. The mean number of inflammatory cells per field in each region of theintestine is summarized in Figure 6.2) Cell proliferationCell proliferation was estimated by <strong>co</strong><strong>un</strong>ting the number of mitosis figures in <strong>en</strong>terocytes on HEslides. The mean number of mitosis figures in the jej<strong>un</strong>um were 2.36 ± 1.64 in the <strong>co</strong>ntrol group, 1.73± 1.35 in the DON treated group, 1.66 ± 1.11 in the FB treated group and 1.91 ± 1.19 in the DON+FBtreated group. In the i<strong>le</strong>um the mean number were 1.75 ± 1.26, 1.78 ± 1.46, 1.62 ± 1.17 and 1.89 ±1.11 for the <strong>co</strong>ntrol group, DON treated group, FB treated group and DON+FB treated group,respectively. A significant decrease (p
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