Funktionelle Analyse von Proteinen der Gpr1/Fun34/yaaH ...

2.6.2 Verwendete Plasmide
Material und Methoden
Tab. 2: Vektoren für S. cerevisiae
Plasmid Genotyp Referenz
p416ADH ADH1prom, Amp r , CYC1ter, CEN6/ARSH4,
URA3
Mumberg et al. (1995)
p426MET25 Amp r , CYC1term, MET25prom, URA3 Mumberg et al. (1994)
pUG30 Amp r ,MET25prom, CYC1term, Kan r , yEGFP3 Güldener and Hege-
mann
YEp351-3HA Amp r , LEU2, lacZ´, 2 µ, MCS/3 x HA-ORF R.J. Schweyen, Wien
YIp352 Amp r , URA3 Hill et al. (1986)
Tab. 3: Vektoren für Y. lipolytica
Plasmid Genotyp Referenz
pG1-P265 Amp r , ARS18, LEU2, GPR1B, C-terminal ab
P265 verkürzt
Gentsch (2003)
pG1-Y256 Amp r , ARS18, LEU2, GPR1B, C-terminal ab
Y265 verkürzt
Gentsch (2003)
pG1-A247 Amp r , ARS18, LEU2, GPR1B, C-terminal ab
A247 verkürzt
Gentsch (2003)
pG1-F242 Amp r , ARS18, LEU2, GPR1B, C-terminal ab
F242 verkürzt
Gentsch (2003)
pG1-dYNAYA Amp r , ARS18, LEU2, GPR1B, YNAYA deletiert Gentsch (2003)
pG2-P265 Amp r , ARS18, LEU2, pGPR1B-GPR1-2,
C-terminal ab P265 verkürzt
Gentsch (2003)
pG2-Y256 Amp r , ARS18, LEU2, pGPR1B-GPR1-2,
C-terminal ab Y256 verkürzt
Gentsch (2003)
pG2-A247 Amp r , ARS18, LEU2, pGPR1B-GPR1-2,
C-terminal ab A247 verkürzt
Gentsch (2003)
pG2-F242 Amp r , ARS18, LEU2, pGPR1B-GPR1-2,
C-terminal ab F242 verkürzt
Gentsch (2003)
pG2-dYNAYA Amp r , ARS18, LEU2, pGPR1B-GPR1-2,
YNAYA deletiert
Gentsch (2003)
pINA237 Amp r , ARS18, LEU2, Tet r Fournier et al. (1993)
pINA443 Amp r , ARS18, URA3 Augstein (2001)
pTSC1-HA Amp r , LEU2, GPR1-3xHA Gentsch (2003)
pUCGlacZF Amp r , GPR1 Augstein (2001)
pYLD103 Amp r , ARS18, LEU2, GPR1-1 Schmid-Berger et al.
(1994)
pYLD203 Amp r , ARS18, LEU2, GPR1-2 Augstein (2001)
pYLG2 Amp r , ARS18, LEU2, pGPR1B-GPR1-2 Augstein (2001)
pYLG3 Amp r , ARS18, LEU2, GPR1B Augstein (2001)
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