EXPORT-ORIENTED MANAGEMENT - IMC Fachhochschule Krems

A. General Concepts and Overview
B. Selected Signalling Pathways such as G-Proteins, Receptor Tyrosine Kinases,
Transforming Growth Factor beta, Wnt, Hedgehog, Notch, Nuclear Factor Kappa
B.
2. Regulation of cell cycle progression and cellular proliferation, Focus on Retinoblastoma
Tumour Suppressor Protein
3. Apoptosis in development and disease, Focus on p53 Tumour Suppressor Protein
4. Membrane Biology and Neural Cell Physiology
5. Direct contact dependent cell communication and the extracellular matrix: General
mechanisms of tissue formation
6. Basic physiology of selected tissues: Muscles, liver, intestine, skin, brain; vision,
hearing, smell
Teaching Methods
Lecture and discussion
Evaluation
Written exams
Genetic Engineering Laboratory
Semester Hours per week Subject code ECTS
Fall 2 GEN_L I 1
Learning Outcomes / Course Objectives
The genetic engineering laboratory will introduce basal techniques in molecular biology –
transformation, miniplasmid preparations, restriction digests, agarose gel
electrophoresis. Additionally calculating buffer compositions will be practiced. Upon
completion of this course, you are expected to:
� be familiar with the introduced techniques in molecular biology and understand
the principles and concepts behind them and are able to apply the knowledge
of these techniques to novel experiments
� be able to calculate buffer compositions and find the necessary information in
manufacturer‘s catalogues
Prerequisites / Level
Basic Laboratory techniques, microbiology, genetic engineering
Course Description
Agarose gel electrophoresis
Preparation of competent cells
Transformation
Miniplasmid Preparation
Restriction digest
DNA quantification
Buffer calculations
FHR-5-0008_Vers.03_Rev.00_2012 527