EXPORT-ORIENTED MANAGEMENT - IMC Fachhochschule Krems

iotech production facilities are presented in detail (support systems and utilities,
production of cell culture media, bioreactors and product holding tanks and containers,
cell separation and disruption systems, ultrafiltration equipment, process
chromatography systems, freeze-drying).
Teaching Methods
Lecture
Evaluation
Final exam
Genetic Engineering Laboratory under GLP
Semester Hours per week Subject code ECTS
Spring 2 GEN_L II 3
Learning Outcomes / Course Objectives
Upon completion of the course the students will
� be able to apply good documentation practice
� understand the application of GLP in a laboratory environment
� use the applied lab techniques (Protein expression, purification,
characterization; DNA purification, restriction, ligation, transformation,
PCR)
Prerequisites / Level
Laboratory Skills, Genetic Engineering Laboratory, GLP/GMP seminar
Course Description
The experiments for the laboratory course ―Genetic Engineering Laboratory under GLP‖
are discussed in the lecture ―GLP/GMP seminar‖. During that seminar a study plan and
protocol sheets are prepared that are then used to document the work in the laboratory
course.
The Genetic Engineering Laboratory will take one week. During this week two
experiments have to be performed:
� Cloning and identification of recombinant clones
� Protein expression and characterization
Teaching Methods
The Genetic Engineering Laboratory is based on the study plan which is prepared during
the preceding GMP / GLP lectures. The students have to design protocol sheets to
document their experimental observations as well as the raw data of their
experiments. In order to meet the goals of the laboratory course the students will be
assisted by the laboratory instructors, who will provide the students with practical tips
and theoretical support. Since safety is a central question in the laboratory, the students
will also have to identify potential hazards in the experimental setup and provide
suggestions on how to minimize these risks during their practical work.
FHR-5-0008_Vers.03_Rev.00_2012 535