EXPORT-ORIENTED MANAGEMENT - IMC Fachhochschule Krems

Recombinant protein production and modification theory
Semester Hours per week Subject code ECTS
Fall 2 PROT_T 3
Learning Outcomes/ Course Objectives
The students will get an overview about the most common Protein expression organisms
which are used in industry and research (E. coli, Yeast, Insect Cells, Mammalian Cells
and in vitro transcription and translation methods). Additionally the most important post-
and cotranslational protein modifications and the corresponding analysis are
discussed. Finally, the student will hear strategies to purify recombinant proteins.
Prerequisites/ Level
Advanced
Course Description:
Overview Expression Organisms in Industry and Research, Novel Expression Systems and
Platforms; E. coli ,Gene Dose, Promoters, Plasmid stability, Signal Sequences and
Secretion, N-end Rule, Strains; Yeast System for Protein Expression, Plasmids, Focus
Pichia pastoris; Insect cells, Plasmids and Systems; Mammalian Cells; In vitro Protein
Production Systems; Protein Purification Strategies; Post and co-translational Modification
of Proteins, Analysis of Protein-Modification
Teaching concept
Students have to work out selected topics (papers provided by the lecturer) in
recombinant protein engineering in small groups.
Evaluation
Presentation and final exam
Research semester preparation
Semester Hours per week Subject code ECTS
Fall 1 PREP 1
Learning Outcomes/ Course Objectives
Students update their curriculum vitas and application forms and are prepared for
interviews.
Prerequisites/ Level
None
Course Description
Specialists from the life science industry are invited to give insights about their work and
various research topics in order for the students to gain a better insight of what possible
fields they could work in. Students learn about different application possibilities and the
do's and don‘ts when writing their application. The importance of cover letters is
discussed and the application documents are updated.
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