THESE Maryse Bonnin Jusserand - Université de Bourgogne
THESE Maryse Bonnin Jusserand - Université de Bourgogne
THESE Maryse Bonnin Jusserand - Université de Bourgogne
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Résultats<br />
Figure 1: Geographical distribution of the 263 O. oeni strains isolated from wine and ci<strong>de</strong>r.<br />
PM G7 H7 A8 B8 C8 bpD8<br />
MWE8 1F8 2G8 3H8 A9 4 MW PM T+ 5 B9 C9 D9 E9 F9 G9<br />
3000<br />
10 B10<br />
2000<br />
1500<br />
1000<br />
500<br />
100<br />
1900pb<br />
PM H9 A10 B10 C10 D10 E10 F10 G10 H10 A11 PM B11 C11 D11 E11 F11 G11 H11 A12<br />
Figure 2: Screening of odc gene by PCR amplification in different Oenococcus oeni strains.<br />
MW: Molecular Weight marker, wells 1 to 5: O. oeni DBA, DIV 5.74, DIV 7.A3, SA.BO 34<br />
and BR14/97. The amplification of odc reveals a single band of 1900 bp.<br />
Cloning of the O. oeni <strong>de</strong>carboxylase gene into E. coli.<br />
Genomic DNA extracted from the O. oeni BR14/97 strain was amplified and a fragment with<br />
approximately 2,200 bp was obtained. As this fragment comprised the complete odc gene it<br />
was directly cloned into pET-28a. The recombinant plasmid obtained named pET-odc was<br />
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