PhylogÃ©nie Et Evolution Du Comportement Social Chez Les Blattes ...

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Ev o l u t i o n d u c o m p o r t e m e n t s o c i a lEx t r a c t i o n1) Dry tissueAdd 180 µL ATL bufferAdd 20 µL proteinaseKWrap in parafilmVortexIncubate at 55°C about 2-2.5 hrs2) Vortex for 15 secAdd 200 µL AL bufferVortexIncubate at 70°C for 10 min3) Add 200 µL 96-100% <strong>Et</strong>ohVortex4) Pipet (500 µL) into Dneasy minicolumns in 2mL tubesCentrifuge = 6000xg (8000 rpm) 1 minDiscard collection tube5) Place columns into new 2mL tubesAdd 500 µL AWI bufferCentrifuge = 6000xg (8000 rpm) 1 minDiscard collection tube6) Place columns into new 2mL tubesAdd 500 µL AWII bufferCentrifuge full speed 3 min7) Place columns into 1,5mL microtubesAdd 200 µL AE bufferLeave for 1 minCentrifuge = 6000xg (8000 rpm) 1 min to elute8) Repeat elution (but with 100 µL AE buffer)Collect elutes together276
ANNEXE IIILeg e n d r e et al . (Bi o l . J. Li n n . So c .)Acc e p t éPhy l o g e n e t i c an a l y s i s of no n -s t e r e o t y p e d be h a v i o u r a ls e q u e n c e s wi t h a su c c e s s i v ee v e n t -pa i r i n g me t h o d
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UFR des Sciences de la VieEcole Doc
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Je remercie toutes les personnes av
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Ta b l e d e s Mat i è r e sRe m e
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Données moléculaires sans les sé
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Ch a p i t r e V : Di s c u s s i o
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Figure 3.10 : Analyse cladistique d
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Li s t e d e s Ta b l e au xTableau
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In t r o d u c t i o nNothing in bi
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Ev o l u t i o n d u c o m p o r t
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I. An a ly s e d u c o n t e x t e
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II. Mat é r i e l s e t m é t h o
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ABCDEGHFJIK
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III. Réversibilité, c o n t r a i
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CHAPITRE IVEv o l u t i o n d e s c
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Page 244 and 245: Ev o l u t i o n d u c o m p o r t
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Page 297 and 298: An n e x e sAbstractA new method is
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Page 303 and 304: An n e x e sMolecular data for all
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Page 307 and 308: An n e x e sThe comparison of the t
Page 309 and 310: An n e x e sDefining and including
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Page 313 and 314: An n e x e sGautier JY, Deleporte P
Page 315 and 316: An n e x e sMcLennan DA, Brooks DR,
Page 317 and 318: An n e x e sWenzel JW. 1993. Applic
Page 319 and 320: An n e x e sTable 1: PCR primers na
Page 321 and 322: An n e x e sTable 4: Phylogenetic a
Page 323 and 324: An n e x e sPhortioeca nimbataP.n.
Page 325 and 326: An n e x e sSchultesia lampyridifor
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Page 329: An n e x e sAnalysis AEublaberus di
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igureEv o l u t i o n d u c o m p o
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FigureEv o l u t i o n d u c o m p
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Figure Ev o l u t i o n d u c o m p
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FigureEv o l u t i o n d u c o m p
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ablesEvo l u t i o n d u c o m p o
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ANNEXE VICom m a n d e s de s an a
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An n e x e sCommandes relatives à
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ANNEXE XIICur r i c u l u m Vi t a
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An n e x e s
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An n e x e s
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Ré s u m éPh y l o g é n i e e t
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PhylogÃ©nie Et Evolution Du Comportement Social Chez Les Blattes ...

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