Phylogénie Et Evolution Du Comportement Social Chez Les Blattes ...

lesAn n e x e sTable 2Sequences of primers and PCR profiles usedGene primer sequence (5'-3') sources12S 12Sai AAA CTA GGA TTA GAT ACC CTA TTA T Simon et al., 199412Sbi AAG AGC GAC GGG CGA TGT GT Simon et al., 199416S 16SAr CGC CTG TTT ATC AAA AAC AT Xiong and Kocher, 199116SF TTA CGC TGT TAT CCC TAA Kambhampati 1995Cytb cytb612 CCA TCC AAC ATC TCC GCA TGA TGA AA Kocher et al., 1989cytb920 CCC TCA GAA TGA TAT TTG GCC TCA Kocher et al., 1989COII Fleu TCT AAT ATG GCA GAT TAG TGC Whiting, 2002Rlys GAG ACC AGT ACT TGC TTT CAG TCA TC Whiting, 2002COI LCO GGT CAA CAA ATC ATA AAG ATA TTG G Folmer et al., 1994HCO TAA ACT TCA GGG TGA CCA AAA AAT CA Folmer et al., 1994mtd6 GGA GGA TTT GGA AAT TGA TTA GTT CC Simon et al., 1994calvin GGR AAR AAW GTT AAR TTW ACT CC Whiting lab, BYU?Ron GGA TCA CCT GAT ATA GCA TT CCC Simon et al., 1994Deep6R2 WCC WAC DGT RAA YAT RTG RTG DGC Whiting lab, BYU?Deep6f2 TTG AYC CWG CWG GDG GDG GNG AYC C Whiting lab, BYU?18S 1F TAC CTG GTT GAT CCT GCC AGT AG Giribet et al., 19961.2F TGC TTG TCT CAA AGA TTA AGC Whiting, 2002b5.0 TAA CCG CAA CAA CTT TAA T Whiting et al., 19972F AGG GTT CGA TTC CGG AGA GGG AGC Hillis and Dixon, 1991b2.9 TAT CTG ATC GCC TTC GAA CCT CT Jarvis et al., 2004a1.0 GGT GAA ATT CTT GGA CCG TC Whiting et al., 19977R GCA TCA CAG ACC TGT TAT TGC Whiting, 2002a3.5 TTG TGC ATG GCC GYT CTT AGT Whiting, 20029R GAT CCT TCC GCA GGT TCA CCT AC Giribet et al., 199628S Rd1.2a CCC SSG TAA TTT AAG CAT ATT A Whiting, 2002Rd3b CCY TGA ACG GTT TCA CGT ACT Jarvis et al., 2004Rd3.2a AGT ACG TGA AAC CGT TCA SGG GT Whiting, 2002Rd4b CCT TGG TCC GTG TTT CAA GAC Jarvis et al., 2004Rd4.2b CCT TGG TCC GTG TTT CAA GAC GG Whiting, 200228SA GAC CCG TCT TGA AGC ACG Whiting et al., 199728SB TCG GAA GGA ACC AGC TAC Whiting et al., 1997Rd4.5a AAG TTT CCC TCA GGA TAG CTG Whiting, 2002Rd7.b1 GAC TTC CCT TAC CTA CAT Whiting, 2002Gene Heat denaturation Annealing extension final extension cyclesAll 94°C (2min) 94°C (1min) 55°C (1min) 72°C (1min 15s) 72°C (7min) 40Cytb 94°C (2min) 94°C (1min) 51.5 to 59.5°C (1min) 72°C (1min 15s) 72°C (7min) 4016S 94°C (2min) 94°C (1min) 50°C (1min) 72°C (1min 15s) 72°C (7min) 40COII 94°C (2min) 94°C (1min) 45 to 53°C (1min) 72°C (1min 15s) 72°C (7min) 40COIa 94°C (2min) 94°C (1min) 49.5 to 57.5°C (1min) 72°C (1min 15s) 72°C (7min) 40COIbc 94°C (2min) 94°C (1min) 50°C (1min) 72°C (1min 15s) 72°C (7min) 4028SB 94°C (2min) 94°C (1min) 50°C (1min) 72°C (1min 15s) 72°C (7min) 40note that PCR where annealing temperature increases were done with an increment of 0.2°C per cycleCOIa = with primers LCO / HCOCOIbc = with primers mtd6 / calvin373