ELECTRONIC POSTER - ismrm

14:30 3751. Targeted New Peptide Based Nanoparticles Toward High EGFR Expressing Cancer
Cells for MRI
Ming-Hung Chen 1 , Gin-Chung Liu 2,3 , Twei-Shiun Jaw 2,4 , Yu-Ting Kuo 2,3 , Chiao-Yun
Chen 2,3 , Yun-Ming Wang 1
1 Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan;
2 Department of Medical Imaging, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan; 3 Department of
Radiology,Faculty of Medicine,College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan;
4 Department of Radiology,Faculty of Medicine,College of Medicine, Kaohsiung Medical University,
Kaohsiung, Taiwan
In this study, the various core sizes of manganese ferrite nanoparticles (MnFe2O4) conjugated with D4 peptide (MnFe2O4-PEG-D4)
were synthesized. The high relaxivity MnFe2O4 nanoparticles were obtained by thermal decomposition of Iron acetylacetonate and
manganese acetylacetonate in hydrophobic solution at high boiling process. The surface of MnFe2O4 nanoparticles were coated with
polyethylene glycol (PEG) and EGFR peptide ligand (D4: Leu-Ala-Arg-Leu-Leu-Thr) to improve their dispersion and ability to target
EGFR. The negative signal enhancement of EGFR expressing cancer cells (SKBR-3 and PC-3) were significantly higher than that of
low EGFR expressing cells (HEK-293).
15:00 3752. In Vivo Detection of a PARACEST Agent in Mouse Brain Tumors
Alex Xuexin Li 1 , Mojmir Suchy 2 , Chunhui Li 1 , Claire Poppe 1 , Joseph Gati 1 , Susan
Meakin 1 , Robert H.E. Hudson 2 , Ravi S. Menon 1 , Robert Bartha 1
1 Robarts Research Institute, London, ON, Canada; 2 The University of Western Ontario
A methodology to detect the on-resonance paramagnetic chemical exchange effects (OPARACHEE) of a PARACEST contrast agent:
Tm 3+ -DOTAM-Glycine (Gly)-Lysine (Lys) in a mouse brain tumor model was developed. The OPARACHEE effect was isolated
from the relaxation effects induced by the PARACEST agent using a control image and an OPARACHEE image. Isolated
OPARACHEE contrast (1-3%) was observed in all animals. Immediately after contrast agent injection OPARACHEE contrast was
observed and maintained at 1~2% in the hour following injection.
Thursday 13:30-15:30 Computer 40
13:30 3753. A Targeted Nanoglobular Manganese(II) Chelate Conjugate for Magnetic
Resonance Cancer Molecular Imaging
Mingqian Tan 1 , Eun-Kee Jeong 2 , Zheng-Rong Lu 1
1 Case Western Reserve University, Cleveland, OH, United States; 2 University of Utah, Salt Lake City, UT,
United States
A peptide targeted nanoglobular Mn(II)-DOTA conjugate was designed and synthesized as MRI contrast agent for cancer molecular
imaging. The target specific contrast agent comprised of 2 peptides and 42 Mn(II) chelates on the surface of the G3 nanoglobule with
a defined structure. The T1 and T2 relaxivities at room temperature are 3.13 and 8.14 mM-1sec-1 per Mn(II) chelate at 3T,
respectively. The targeted nanoglobular contrast agent specifically bound to tumor tissue and resulted in significant tumor contrast
enhancement in tumor-bearing mice as compared to a non-targeted control at a dose as low as 0.03 mmol-Mn/kg .
14:00 3754. Multi-Functional Nanocontrast Agents for In Vivo Probing on Non-Small Cell Lung
Cancer in MR and Optical Molecular Imaging
Ching-Tang Chen 1 , Chia-Hao Su 2 , Yi-Chien Lu 1 , Ang Yuan 3 , Jyh-Horng Chen 1
1 Interdisciplinary MRI/MRS Lab, Department of Electrical Engineering, National Taiwan University, Taipei,
Taiwan, Taiwan; 2 Center for Translational Research in Biomedical Science, Chang Gung Memorial Hospital,
Kaohsiung, Taiwan; 3 Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan,
Taiwan
Molecular imaging has become an indispensable technology in cancer research and clinical use. The goal of this study is to combine
magnetic resonance imaging and optical imaging system with multifunctional contrast agent to detect xenograft non-small cell lung
cancer (NSCLC) murine model.
High temperature solution phase reaction led to 8 nm ultrasmall superparamagnetic iron oxide (USPIO, Fe 3 O 4 ), and the CdS-capped
CdTe x Se 1-x alloyed quantum dot was synthesized to near-IR emitting nanoparticles. Aqueous iron oxide and near-IR quantum dot
nanoparticles were conjugated with anti-epideremal growth factor receptor (EGFR) antibody as the biomedical probe to detect the
NSCLC tumor. Variation of T2 relaxation time was obtained from MRI for nano-contrast agent quantification. Prussian blue staining
imaging showed different targeting efficiency in A549 and CL1-0 in vitro. T2 and T2* MR imaging showed significant signal
decrease (>30%) in vivo. It was proved caused by nano-probe targeting by using both histological cytochemistry staining.
Multifunctional nanocontrast agent could hopefully not only serves as cancer detection and treatment but also used to predict disease
prognosis in the future.