ELECTRONIC POSTER - ismrm

Thursday 13:30-15:30 Computer 7
13:30 3272. Detection of Early Response to Temozolomide Treatment in Brain Tumors Using
Hyperpolarized 13 C MR Metabolic Imaging
Ilwoo Park 1,2 , Myriam Chaumeil 2 , Tomoko Ozawa 3 , Sabrina M. Ronen 1,2 , Daniel B.
Vigneron 1,2 , C. David James 3 , Sarah J. Nelson 1,2
1 Joint Graduate group in Bioengineering, University of California San Francisco/Berkeley, San Francisco, CA,
United States; 2 Surbeck Laboratory of Advanced Imaging, Department of Radiology and Biomedical Imaging,
University of California San Francisco, San Francisco, CA, United States; 3 Brain Tumor Research Center,
Department of Neurological Surgery, University of California San Francisco, San Francisco, CA, United States
We have demonstrated the feasibility of using DNP hyperpolarized 13 C 1 -pyruvate to detect early response to Temozolomide treatment
in an orthotopic human glioblastoma xenograft model in rat brain. The 13 C data from the treated rats showed the ability to detect
altered tumor metabolism as early as one day after TMZ treatment initiation, while the tumor volume from T1 post-Gd imaging
showed the first sign of reduction at the 8th day after the initiation of treatment.
14:00 3273. MR Technical Developments for Clinical Hyperpolarized 13 C-Pyruvate Studies in
Prostate Cancer Patients
Peder E. Z. Larson 1 , James Tropp 2 , Albert P. Chen 3 , Paul Calderon 2 , Simon Hu 1 , Galen
Reed 1 , Sarah J. Nelson 1 , John Kurhanewicz 1 , Ralph Hurd 2 , Daniel B. Vigneron 1
1 Radiology and Biomedical Imaging, University of California - San Francisco, San Francisco, CA, United
States; 2 Applied Science Laboratory, GE Healthcare, Menlo Park, CA, United States; 3 Applied Science
Laboratory, GE Healthcare, Toronto, Ontario, Canada
We have developed and tested custom hardware and methods for future prostate cancer patient studies with hyperpolarized 13 C-
pyruvate, including 13 C coils for prostate imaging, clean room dissolution DNP system, and hyperpolarized 13 C pulse sequences.
14:30 3274. Detection of Pentose Phosphate Pathway Flux Using Hyperpolarized [1-
13 C]Gluconolactone in Mouse Livers
Karlos X. Moreno 1 , Crystal E. Harrison 1 , Matthew E. Merritt 1 , Zoltan Kovacs 1 , Zengdun
Shi 2 , Don C. Rockey 2 , A Dean Sherry 1 , Craig R. Malloy 1,2
1 Advanced Imaging Research Center, Univ of TX Southwestern Med Ctr, Dallas, TX, United States; 2 Internal
Medicine, Univ of TX Southwestern Med Ctr, Dallas, TX, United States
Pentose phosphate pathway flux was studied using hyperpolarized δ-[1- 13 C]gluconolactone injected into an isolated perfused mouse
liver. Control livers produced a significant amount of H 13 CO 3 - , a product indicative of pentose phosphate pathway flux and [1-
13 C]gluconate. Hydrogen peroxide damaged livers also produced H 13 CO 3 - and [1- 13 C]gluconate, though the bicarbonate was at lower
amounts than the control. CCl 4 treated livers did not produce any observable H 13 CO 3 - , but [1- 13 C]gluconate was produced. These
studies show that the lactone is incorporated within the hepatocyte, phosphorylated and metabolized through the pentose phosphate
pathway.
15:00 3275. Effect of the Monocarboxylate Transporter Inhibitor α-Cyano-4-Hydroxy-
Cinnamate on In Vivo Hyperpolarized MR Spectroscopic Imaging with [1- 13 C]Pyruvate
Simon Hu 1 , Robert Bok 1 , Asha Balakrishnan 2 , Andrei Goga 2 , John Kurhanewicz 1 , Daniel
B. Vigneron 1
1 Dept. of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, CA,
United States; 2 Dept. of Medicine, Division of Hematology/Oncology, University of California, San Francisco,
San Francisco, CA, United States
Development of hyperpolarized technology utilizing dynamic nuclear polarization has enabled the measurement of 13 C metabolism in
vivo at very high SNR. The most researched agent for in vivo applications has been [1- 13 C]pyruvate. In this project, the role of cell
membrane transport on the conversion of [1- 13 C]pyruvate to [1- 13 C]lactate and [1- 13 C]alanine in vivo was investigated by using the
monocarboxylate transporter inhibitor α-cyano-4-hydroxy-cinnamate. Reduced hyperpolarized alanine and lactate were detected after
α-cyano-4-hydroxy-cinnamate administration, indicating that this inhibitor approach can be used in vivo to investigate the transport
and intracellular conversion of [1- 13 C]pyruvate.
Hyperpolarized Carbon-13 & Other Nuclei II