ELECTRONIC POSTER - ismrm

14:30 4528. Localized in Vivo 1 H NMR Spectroscopy of the Rat Brain at 16.4T
Sung-Tak Hong 1 , Dávid Zsolt Balla 1 , Gunamony Shajan 1 , Changho Choi 2 , Kamil
Ugurbil 3 , Rolf Pohmann 1
1 High-Field Magnetic Resonance Center , Max-Planck Institute for Biological Cybernetics, Tuebingen, Baden-
Wuerttemberg, Germany; 2 Advanced Imaging Research Center, University of Texas Southwestern Medical
Center, Dallas, TX, United States; 3 Center for Magnetic Resonance Research, University of Minnesota,
Minneapolis, MN, United States
In vivo 1H NMR spectroscopy has several problems including narrow spectral dispersion and low SNR. Increasing a static field
strength could alleviate these problems, providing feasibilities of quantifying metabolites not observable at lower field strength. The
acquisition of an enhanced neurochemical profile was obtained by minimizing TE up to 1.7 ms in a STEAM sequence at 16.4T. The
technique demonstrated possibilities of quantification of additional metabolites, acetate and ethanolamine, in the rat brain in vivo.
15:00 4529. A Multinuclear NMR Study of Glucose Metabolismin Thiamine-Deficient
Cerebellar Granule Cells: New Mechanistic Insights
Abolghasem Mohammadi 1 , Claudia Zwingmann 1
1 Département de médicine, Université de Montréal, Montreal, Quebec, Canada
Wernicke's encephalopathy is a neurological disorder which is characterized by disturbances in consciousness and region-selective
brain lesions. Brain damage is associated with a deficiency in thiamine, an essential vitamine in carbohydrate metabolism.
Multinuclear NMR spectroscopy was used to assess carbon fluxes and cellular energy state of thiamine-deficient cultured rat
cerebellar granule neurons. The data show that neuronal energy failure and death likely result from a primary impairment of neuronal
a-KGDH causing impaired carbon flux from glucose/pyruvate through PDH, decreased catabolism of glutamine, and lactate
accumulation. Under hyperglycemic conditions, however, alternative explanations to the lactic acidosis hypothesis have to be
considered.
Thursday 13:30-15:30 Computer 89
13:30 4530. Taurine Change in Visual Cortex of Neonatal Monocular Enucleated Rat: A Proton
MRS Study
April M. Chow 1,2 , Iris Y. Zhou 1,2 , Shu Juan Fan 1,2 , Kannie W.Y. Chan 1,2 , Kevin C. Chan 1,2 ,
Ed X. Wu 1,2
1 Laboratory of Biomedical Imaging and Signal Processing, The University of Hong Kong, Pokfulam, Hong
Kong SAR, China; 2 Department of Electrical and Electronic Engineering, The University of Hong Kong,
Pokfulam, Hong Kong SAR, China
Neonatal monocular enucleation has been used to study developmental mechanisms underlying visual perception and the cross-modal
changes in the central nervous system caused by early loss of the visual input. In this study, we demonstrated that alteration in the
metabolism of taurine in visual cortex accompanied with neonatal monocular enucleation could be monitored using 1 H MRS at 7 T.
The change in taurine signal with respect to creatine signal may possibly due to the increased taurine signal in the right control visual
cortex, likely caused by the plasticity resulted from recruitment of resources to the remaining left eye for adaptation.
14:00 4531. Simultaneously Measuring Glucose Transport Constants and Cerebral Metabolic
Rate of Glucose by in Vivo 1 H MRS in the Rat Brain
Fei Du 1,2 , Yi Zhang 1 , Xiao-Hong Zhu 1 , Wei Chen 1
1 Radiology, Center for Magnetic Resonance Research, University of Minnesota, Minneapolis, MN, United
States; 2 Psychiatry, McLean Hospital, Harvard University, Belmont, MA, United States
The basal brain activity and function depends upon a constant supply of glucose through the specific glucose transport mechanism
mediated by transporter molecules, referred to as the blood-brain barierr (BBB). Therein the noninvasive method to reliably measure
glucose cerebral metabolic rate and transport constants are of importance for understanding underlying glucose transport mechanism
and energy consumption in the various physiological or pathological conditions. For instance, in our previous studies, we observed
that compared to the light anesthesia (2% v-v isofulorane), brain glucose concentration significantly decreased although cerebral
metabolic rate of glucose (CMRglc) decreased 37% at the iso-electric condition. This observation of glucose reduction seemingly
contradicted with other studies showing a decreased brain glucose concentration accompanied by the increased CMR glc due to the
elevated stimulations. This apparent discrepancy can be explained by the changes of blood plasma glucose concentrations, which were
found to be substantially decreased under the iso-electric conditions. Another possible reason is alterations of glucose transport
constants (K T and T max ). It was reported that pentobarbital reduced blood-brain glucose transfer in the rat brain and the glucose
transport constants decreased compared to the awaken condition. Therefore, the aim of the current study is to build-up a noninvasive
method to reliably and simultaneously measure CMR glc and transport constants for fully understanding brain glucose concentration
changes with alterations of anesthesia depth. The method was introduced and implemented by simultaneously measuring plasma and
brain tissue glucose concentration time courses after stopping glucose infusion.